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논문 기본 정보
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제9호
- 발행연도
- 2009.1
- 수록면
- 1,034 - 1,040 (7page)
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초록· 키워드
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Porin proteins of Gram-negative bacteria are outer
membrane proteins that act as receptors for bacteriophages
and are involved in a variety of functions like solute
transport, pathogenesis, and immunity. Salmonella enterica
serovar Typhi (S. Typhi), a Gram-negative bacterium, is
the causative agent of typhoid fever. Porins of S. Typhi
have been shown to have a potential role in diagnostics
and vaccination. In the present study, the major outer
membrane proteins OmpF and OmpC from S. Typhi were
cloned in pQE30UA vector and expressed in E. coli. The
immunogenic nature of the recombinant porin proteins
were evaluated by ELISA by raising hyperimmune sera in
Swiss Albino mice with three different adjuvants (i.e.,
Freund’s adjuvant and two human-compatible adjuvants
like montanide and aluminium hydroxide gel) and proved
to be immunogenic. The recombinant OmpF and OmpC
generated in this work may be used for further studies for
vaccination and diagnostics.
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참고문헌 (21)
참고문헌 신청
Arokiasamy, A. / 2004 / Folding and structural stability of OmpC from Salmonella Typhi: Role of LPS and environment / Curr. Sci 87 : 197 ~ 202
Aron, L. / 1993 / Lipopolysaccharide-independent radioimmunoprecipitation and identification of structural and in vivo induced immunogenic surface proteins of Salmonella Typh
Calderon, I. / 1986 / Antibodies to porin antigens of Salmonella Typhi induced during typhoid infection in humans / Infect. Immun 52 : 209 ~ 212
Chatfield, S. N. / 1991 / Role of ompR-dependent genes in Salmonella Typhimurium virulence: Mutants deficient in both ompC and ompF are attenuated in vivo / Infect. Immun 59
Contreras, I. / 1995 / Heterologous expression of Escherichia coli porin genes in Salmonella Typhi Ty2: Regulation by medium osmolarity, temperature and oxygen availability /
Aron, L. / 1993 / Lipopolysaccharide-independent radioimmunoprecipitation and identification of structural and in vivo induced immunogenic surface proteins of Salmonella Typh
Calderon, I. / 1986 / Antibodies to porin antigens of Salmonella Typhi induced during typhoid infection in humans / Infect. Immun 52 : 209 ~ 212
Chatfield, S. N. / 1991 / Role of ompR-dependent genes in Salmonella Typhimurium virulence: Mutants deficient in both ompC and ompF are attenuated in vivo / Infect. Immun 59
Contreras, I. / 1995 / Heterologous expression of Escherichia coli porin genes in Salmonella Typhi Ty2: Regulation by medium osmolarity, temperature and oxygen availability /
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