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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제3호
- 발행연도
- 2015.1
- 수록면
- 375 - 380 (6page)
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초록· 키워드
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Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30oC. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition.
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참고문헌 (24)
참고문헌 신청
Anthony C. / 1986 / Bacterial oxidation of methane and methanol / Adv. Microb. Physiol 27 : 113 ~ 210
Burnham A / 2011 / Life-cycle greenhouse gas emissions of shale gas, natural gas, coal, and petroleum / Environ. Sci. Technol 46 : 619 ~ 627
Burrows KJ / 1984 / Substrate specificities of the soluble and particulate methane monooxygenases of Methylosinus trichosporium OB3b / J. Gen. Microbiol 130 : 3327 ~ 3333
Casey PS / 1994 / Selective oxidation of methane to methanol at high pressures / Ind. Eng. Chem. Res 33 : 1120 ~ 1125
Conrado RJ / 2014 / Envisioning the bioconversion of methane to liquid fuels / Science 343 : 621 ~ 623
Burnham A / 2011 / Life-cycle greenhouse gas emissions of shale gas, natural gas, coal, and petroleum / Environ. Sci. Technol 46 : 619 ~ 627
Burrows KJ / 1984 / Substrate specificities of the soluble and particulate methane monooxygenases of Methylosinus trichosporium OB3b / J. Gen. Microbiol 130 : 3327 ~ 3333
Casey PS / 1994 / Selective oxidation of methane to methanol at high pressures / Ind. Eng. Chem. Res 33 : 1120 ~ 1125
Conrado RJ / 2014 / Envisioning the bioconversion of methane to liquid fuels / Science 343 : 621 ~ 623
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