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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제5호
- 발행연도
- 2012.1
- 수록면
- 600 - 606 (7page)
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Studies into the cell death program termed apoptosis have resulted in new information regarding how cells control and execute their own demise, including insights into the mechanism by which death-preventing factors can inhibit Bax-induced caspase activation. We investigated high temperature stress-induced cell death in Brassica rapa.
Using a yeast functional screening from a Brassica rapa cDNA library, the BH5-127 EST clone encoding an apoptotic suppressor peptide was identified. However, a phylogenic tree showed that BH5-127 clusters within a clade containing SUMO-1 (Small Ubiquitin-like Modifier-1). BH5-127 was confirmed similar to have function to SUMO-1 as Fas suppression. Expression of BH5-127showed that substantial suppression of cell death survived on SD-galactose-Leu--Ura- medium. The results suggest that BrSE (Brassica rapa Sentrin EST, BH5-127) is one of the important regulatory proteins in programming cell death, especially in the seedling stage of Chinese cabbage.
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참고문헌 (20)
참고문헌 신청
Angadi, S. V. / 2000 / Response of three Brassica species to high temperature stress during reproductive growth / Can. J. Plant Sci 80 : 693 ~ 701
Chen, S. R. / 2003 / Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae / Free Radic. Biol. Med 34 : 1315 ~ 1325
Eswaran, N. / 2010 / Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas / BMC Biotechnol 10 : 23 ~
Hanania, U. / 1999 / Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death / Plant J 19 : 533 ~ 541
Isbat, M. / 2009 / A Bax inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco / J. Plant Physiol 166
Chen, S. R. / 2003 / Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae / Free Radic. Biol. Med 34 : 1315 ~ 1325
Eswaran, N. / 2010 / Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas / BMC Biotechnol 10 : 23 ~
Hanania, U. / 1999 / Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death / Plant J 19 : 533 ~ 541
Isbat, M. / 2009 / A Bax inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco / J. Plant Physiol 166
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