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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제7호
- 발행연도
- 2009.1
- 수록면
- 713 - 717 (5page)
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초록· 키워드
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The dependence of the catalytic properties of horseradish
peroxidase on the structural changes of ionic liquids was
investigated with two water-miscible ionic liquids, N-butyl-3-
methypyridinium tetrafluoroborate ([BMPy][BF4]) and 1-butyl-
3-methylimidazolium methylsulfate ([BMIM][MeSO4]), each of
which shares an anion (BF4
-) or a cation (BMIM+) with 1-butyl-
3-methylimidazolium tetrafluoroborate ([BMIM][BF4]),
respectively. The oxidation of guaiacol (2-methoxyphenol)
with H2O2 was used as a model reaction. In order to minimize
the effect of solution viscosity on the kinetic constants of
the enzymatic catalysis, the enzymatic reactions for the
kinetic study were performed in water-ionic liquid mixtures
containing 25% (v/v) ionic liquid at maximum. Similarly
to the previously reported results for [BMIM][BF4], as the
concentration of [BMPy][BF4] increased, the Km value
increased with a decrease in the kcat value: the Km value
increased markedly from 2.8 mM in 100% water to
12.6 mM in 25% (v/v) ionic liquid, indicating that ionic
liquid significantly weakens the binding affinity of
guaiacol to the enzyme. On the contrary, [BMIM][MeSO4]
decreased the Km value to 1.4 mM in 25% (v/v) ionic
liquid. [BMIM][MeSO4] also decreased kcat more than 3-
folds [from 13.8 s-1 in 100% water to 4.1 s-1 in 25% (v/v)
ionic liquid]. These results indicate that the ionic liquids
interact with the enzyme at the molecular level as well as
at a macroscopic thermodynamic scale. Specifically, the
anionic component of the ionic liquids influenced the
catalysis of horseradish peroxidase in different ways.
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참고문헌 (21)
참고문헌 신청
Al-Kassim, L. / 1994 / Enzymatic removal of selected aromatic contaminants from wastewater by a fungal peroxidase from Coprinus macrorhizus in batch reactor / J. Chem. Tech.
CHAKRABORTY, JOYDEEP / 2006 / Isolation of a Pseudomonas sp. Capable of Utilizing 4-Nonylphenol in the Presence of Phenol / Journal of Microbiology and Biotechnology 16 (11)
Ghan, H. / 2004 / Enzyme-catalyzed polymerization of phenols within polyelectrolyte microcapsules / Macromolecules 37 : 4519 ~ 4524
Halliwell, B. / 1976 / Hydroxylation of p-coumaric acid by horseradish peroxidase / Biochem. J 153 : 513 ~ 518
Hinckley, G. / 2002 / Oxidative enzymes possess catalytic activity in systems with ionic liquids / Biotechnol. Lett 24 : 2083 ~ 2087
CHAKRABORTY, JOYDEEP / 2006 / Isolation of a Pseudomonas sp. Capable of Utilizing 4-Nonylphenol in the Presence of Phenol / Journal of Microbiology and Biotechnology 16 (11)
Ghan, H. / 2004 / Enzyme-catalyzed polymerization of phenols within polyelectrolyte microcapsules / Macromolecules 37 : 4519 ~ 4524
Halliwell, B. / 1976 / Hydroxylation of p-coumaric acid by horseradish peroxidase / Biochem. J 153 : 513 ~ 518
Hinckley, G. / 2002 / Oxidative enzymes possess catalytic activity in systems with ionic liquids / Biotechnol. Lett 24 : 2083 ~ 2087
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