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We evaluated a commercial biopreparation of plantgrowth-promoting rhizobacteria (PGPR) strains Bacillus subtilisGB03 and B. amyloliquefaciens IN937a formulated with thecarrier chitosan (BioYield) for its capacity to elicit growthpromotion and induced systemic resistance against infectionby Cucumber Mosaic Virus (CMV) and Pseudomonas syringaepv. tomato DC3000 in Arabidopsis thaliana. The biopreparationpromoted plant growth of Arabidopsis hormonal mutants,which included auxin, gibberellic acid, ethylene, jasmonate,salicylic acid, and brasinosteroid insensitive lines as well aseach wild-type. The biopreparation protected plants againstCMV based on disease severity in wild-type plants. However,virus titre was not lower in control plants and those treatedtolerance rather than resistance against CMV. Interestingly,the biopreparation induced resistance against CMV in NahGplants, as evidenced by both reduced disease severity andvirus titer. The biopreparation also elicited induced resistanceagainst P. syringae pv. tomato in the wild-type but not inNahG transgenic plants, which degrade endogenous salicylicacid, indicating the involvement of salicylic acid signaling.growth promotion by mechanisms that are diferent from knownhormonal signaling pathways. In addition, the mechanism forelicitation of induced resistance by PGPR may be pathogen-dependent. Colectively, the two-Bacili strain mixture can beutilized as a biological inoculant for both protection of plantagainst bacterial and viral pathogens and enhancement ofplant growth.

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