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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 297 - 305 (9page)
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The use of peroxidase in the nitration of phenols is gaining interest as compared with traditional chemical reactions. We investigated the kinetic characteristics of phenol nitration catalyzed by horseradish peroxidase (HRP) in an aqueous-organic biphasic system using n-butanol as the organic solvent and NO2 - and H2O2 as substrates. The reaction rate was mainly controlled by the reaction kinetics in the aqueous phase when appropriate agitation was used to enhance mass transfer in the biphasic system. The initial velocity of the reaction increased with increasing HRP concentration. Additionally, an increase in the substrate concentrations of phenol (0–2 mM in organic phase) or H2O2 (0–0.1 mM in aqueous phase) enhanced the nitration efficiency catalyzed by HRP. In contrast, high concentrations of organic solvent decreased the kinetic parameter Vmax/Km. No inhibition of enzyme activity was observed when the concentrations of phenol and H2O2 were at or below 10 mM and 0.1 mM, respectively. On the basis of the peroxidase catalytic mechanism, a double-substrate ping-pong kinetic model was established. The kinetic parameters were Km H2O2 = 1.09 mM, Km PhOH = 9.45 mM, and Vmax = 0.196 mM/min. The proposed model was well fit to the data obtained from additional independent experiments under the suggested optimal synthesis conditions. The kinetic model developed in this paper lays a foundation for further comprehensive study of enzymatic nitration kinetics.
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참고문헌 (27)
참고문헌 신청
Anni H / 1992 / Mechanism of action of peroxidases / Met. Ions Biol. Syst 28 : 219 ~ 241
Baker PR / 2005 / Fatty acid transduction of nitric oxide signaling: multiple nitrated unsaturated fatty acid derivatives exist in human blood and urine and serve as endogeno
Bruckdorfer KR / 2001 / The nitration of proteins in platelets / C. R. Acad. Sci. III 324 : 611 ~ 615
Budde CL / 2001 / Enzymatic nitration of phenols / J. Mol. Catal. B Enzym 15 : 55 ~ 64
Casella L / 2010 / Biocatalysis Based on Heme Peroxidases: Peroxidases as Potential Industrial Biocatalysts / Springer : 111 ~ 153
Baker PR / 2005 / Fatty acid transduction of nitric oxide signaling: multiple nitrated unsaturated fatty acid derivatives exist in human blood and urine and serve as endogeno
Bruckdorfer KR / 2001 / The nitration of proteins in platelets / C. R. Acad. Sci. III 324 : 611 ~ 615
Budde CL / 2001 / Enzymatic nitration of phenols / J. Mol. Catal. B Enzym 15 : 55 ~ 64
Casella L / 2010 / Biocatalysis Based on Heme Peroxidases: Peroxidases as Potential Industrial Biocatalysts / Springer : 111 ~ 153
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