지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제11호
- 발행연도
- 2013.1
- 수록면
- 1,586 - 1,597 (12page)
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Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both glycolysis and glutaminolysis were studied through the kinetic analyses of major extracellular metabolites concerning the fast and slow cell growth stages, respectively, as well as the time-course profiles of intracellular metabolites. In three cultures showing distinct antibody productivities, the amino acid metabolism and energy state were further examined. Both the transition of lactate from production to consumption and steady intracellular pools of pyruvate and lactate were observed to be correlated with efficient energy regulation. In addition, an efficient utilization of amino acids as the replenishment for the TCA cycle was also found in the cultures with upregulated energy metabolism. It was further revealed that the inefficient energy regulation would cause low cell productivity based on the comparative analysis of cell growth and productivity in cultures having distinct energy regulation.
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참고문헌 (23)
참고문헌 신청
[학술지(정기간행
Aggarwal S / 2011 / What's fueling the biotech engine-2010 to 2011 / Nat. Biotechnol 29 : 1083 ~ 1089
Alete DE / 2005 / Proteomic analysis of enriched microsomal fractions from GS-NS0 murine myeloma cells with varying secreted recombinant monoclonal antibody productivities /
Altamirano C / 2006 / Considerations on the lactate consumption by CHO cells in the presence of galactose / J. Biotechnol 125 : 547 ~ 556
Chen F / 2012 / Insight into the roles of hypoxanthine and thymidine on cultivating antibody-producing CHO cells: cell growth, antibody production and long-term stability / A
Aggarwal S / 2011 / What's fueling the biotech engine-2010 to 2011 / Nat. Biotechnol 29 : 1083 ~ 1089
Alete DE / 2005 / Proteomic analysis of enriched microsomal fractions from GS-NS0 murine myeloma cells with varying secreted recombinant monoclonal antibody productivities /
Altamirano C / 2006 / Considerations on the lactate consumption by CHO cells in the presence of galactose / J. Biotechnol 125 : 547 ~ 556
Chen F / 2012 / Insight into the roles of hypoxanthine and thymidine on cultivating antibody-producing CHO cells: cell growth, antibody production and long-term stability / A
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