지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제9호
- 발행연도
- 2013.1
- 수록면
- 1,293 - 1,303 (11page)
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초록· 키워드
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Antibiotic resistance of soilborne Acinetobacter species has been poorly explored. In this study,norfloxacin resistance of a soil bacterium, Acinetobacter oleivorans DR1, was investigated. The frequencies of mutant appearance of all tested non-pathogenic Acinetobacter strains were lower than those of pathogenic strains under minimum inhibitory concentration (MIC). When the quinolone-resistance-determining region of the gyrA gene was examined, only one mutant (His78Asn) out of 10 resistant variants had a mutation. Whole transcriptome analysis using a RNA-Seq demonstrated that genes involved in SOS response and DNA repair were significantly up-regulated by norfloxacin. Determining the MICs of survival cells after norfloxacin treatment confirmed some of those cells were indeed persister cells. Ten colonies,randomly selected from among those that survived in the presence of norfloxacin, did not exhibit increased MIC. Thus, both the low mutation frequency of the target gene and SOS response under norfloxacin suggested that persister formation might contribute to the resistance of DR1 against norfloxacin. The persister frequency increased without a change in MIC when stationary phase cells, low growth rates conditions, and growth-deficient dnaJ mutant were used. Taken together, our comprehensive approach, which included mutational analysis of the target gene, persister formation assays, and RNA sequencing, indicated that DR1 survival when exposed to norfloxacin is related not only to target gene mutation but also to persister formation, possibly through up-regulation of the SOS response and DNA repair genes.
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참고문헌 (22)
참고문헌 신청
Andriole VT. / 2005 / The quinolones: past, present, and future / Clin. Infect. Dis 41 : 113 ~ 119
Aranda J / 2011 / Acinetobacter baumannii RecA protein in repair of DNA damage, antimicrobial resistance, general stress response, and virulence / J. Bacteriol 193 : 3740 ~ 3
Berenstein D. / 1986 / Prophage induction by ultraviolet light in Acinetobacter calcoaceticus / J. Gen. Microbiol 132 : 2633 ~ 2636
Bergogne-Berezin E / 1996 / Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features / Clin. Microbiol. Rev 9 : 148 ~ 165
Bernier SP / 2013 / Starvation, together with the SOS response, mediates high biofilm-specific tolerance to the fluoroquinolone ofloxacin / PLoS Genet 9 : e1003144 ~
Aranda J / 2011 / Acinetobacter baumannii RecA protein in repair of DNA damage, antimicrobial resistance, general stress response, and virulence / J. Bacteriol 193 : 3740 ~ 3
Berenstein D. / 1986 / Prophage induction by ultraviolet light in Acinetobacter calcoaceticus / J. Gen. Microbiol 132 : 2633 ~ 2636
Bergogne-Berezin E / 1996 / Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features / Clin. Microbiol. Rev 9 : 148 ~ 165
Bernier SP / 2013 / Starvation, together with the SOS response, mediates high biofilm-specific tolerance to the fluoroquinolone ofloxacin / PLoS Genet 9 : e1003144 ~
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