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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제10호
- 발행연도
- 2009.1
- 수록면
- 1,223 - 1,229 (7page)
이용수
초록· 키워드
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To construct a new recombinant strain of Bacillus velezensis
that has antifungal and insecticidal activity via the
expression of the insecticidal Bacillus thuringiensis crystal
protein, a B. thuringiensis expression vector (pHT1K-1Ac)
was generated that contained the B. thuringiensis cry1Ac
gene under the control of its endogenous promoter in a
minimal E. coli-B. thuringiensis shuttle vector (pHT1K).
This vector was introduced into a B. velezensis isolate that
showed high antifungal activities against several plant
diseases, including rice blast (Magnaporthe grisea), rice
sheath blight (Rhizotonia solani), tomato gray mold (Botrytis
cinerea), tomato late blight (Phytophthora infestans), and
wheat leaf rust (Puccinia recondita), by electroporation.
The recombinant B. velezensis strain was confirmed by
PCR using cry1Ac-specific primers. Additionally, the
recombinant strain produced a protein approximately
130 kDa in size and parasporal inclusion bodies similar to
B. thuringiensis. The in vivo antifungal activity assay
demonstrated that the activity of the recombinant B.
velezensis strain was maintained at the same level as that
of wild-type B. velezensis. Furthermore, it exhibited high
insecticidal activity against a lepidopteran pest, Plutella
xylostella, although its activity was lower than that of a
recombinant B. thuringiensis strain, whereas wild-type B.
velezensis strain did not show any insecticidal activity.
These results suggest that this recombinant B. velezensis
strain can be used to control harmful insect pests and
fungal diseases simultaneously in one crop.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)]
Alcantara, E. P. / 2004 / Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers / Arch. Insect Biochem. Physiol 55 : 169 ~ 177
Bafana, A. / 2008 / Azoreductase and dye detoxification activities of Bacillus velezensis strain AB / Appl. Microbiol. Biotechnol 77 : 1139 ~ 1144
Bora, R. S. / 1994 / Introduction of a lepidopteran-specific insecticidal crystal protein gene of Bacillus thuringiensis subsp. kurstaki by conjugal transfer into a Bacillus
CHOI, GYUNG JA / 2007 / Antifungal Activities of Bacillus thuringiensis Isolates on Barley and CucumberPowdery Mildews / Journal of Microbiology and Biotechnology 17 (12) : 2
Alcantara, E. P. / 2004 / Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers / Arch. Insect Biochem. Physiol 55 : 169 ~ 177
Bafana, A. / 2008 / Azoreductase and dye detoxification activities of Bacillus velezensis strain AB / Appl. Microbiol. Biotechnol 77 : 1139 ~ 1144
Bora, R. S. / 1994 / Introduction of a lepidopteran-specific insecticidal crystal protein gene of Bacillus thuringiensis subsp. kurstaki by conjugal transfer into a Bacillus
CHOI, GYUNG JA / 2007 / Antifungal Activities of Bacillus thuringiensis Isolates on Barley and CucumberPowdery Mildews / Journal of Microbiology and Biotechnology 17 (12) : 2
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