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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제12호
- 발행연도
- 2008.1
- 수록면
- 1,953 - 1,957 (5page)
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초록· 키워드
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Recombinant Lactobacillus strains have been constructed
using gene splicing by overlap extension (SOE). Primers
were designed of which one end of an amplified product
contained complementary sequences for an end of other
amplified fragment. For efficient matching, we used an
asymmetric PCR step that was effective at generating an
excess of strands that would anneal in the final PCR.
CP12, a recombinant fragment consisting of the integrase
gene and attachment site of the bacteriophage A2, was
constructed and inserted into the genome of Lactobacillus
casei ATCC 393, yielding Lb. casei ATCC 393::XCP12.
Another recombinant Lb. casei strain was constructed,
where the egfp gene was a part of the construction. The
EGFP produced from Lb. casei ATCC 393::XCEGFP14
was detected by Western blot hybridization. This simple
and widely applicable approach has significant advantages
over standard recombinant DNA techniques for Lactobacillus
species.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
De Ruyter, P. G. / 1996 / Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl / Environ. Microbiol 62 : 3662 ~ 3667
Flicek, P. / 2006 / Using several pair-wise informant sequences for de novo prediction of alternatively spliced transcripts / Genome Biol 7
Gao, F. S. / 2006 / Reconstruction of a swine SLA-I protein complex and determination of binding nonameric peptides derived from the foot-and-mouth disease virus. Vet. Immuno
Gosalbes, M. J. / 2000 / Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei. Appl. Environ / Microbiol 66 : 4822 ~ 4828
Horton, R. M. / 1989 / Engineering hybrid genes without the use of restriction enzymes:Gene splicing by overlap extension.Gene 77:61-68 / Gene 77 : 61 ~ 68
Flicek, P. / 2006 / Using several pair-wise informant sequences for de novo prediction of alternatively spliced transcripts / Genome Biol 7
Gao, F. S. / 2006 / Reconstruction of a swine SLA-I protein complex and determination of binding nonameric peptides derived from the foot-and-mouth disease virus. Vet. Immuno
Gosalbes, M. J. / 2000 / Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei. Appl. Environ / Microbiol 66 : 4822 ~ 4828
Horton, R. M. / 1989 / Engineering hybrid genes without the use of restriction enzymes:Gene splicing by overlap extension.Gene 77:61-68 / Gene 77 : 61 ~ 68
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