지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 292 - 299 (8page)
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In this study, we analyzed the changes in fungal populations of red-pepper fields employing eco-friendly farming methods, such as microbial agents and crop rotation, by using polymerase chain reactions coupled with denaturing gradient gel electrophoresis (PCR-DGGE). Primer specific for fungi were used to determine the contribution of domains to the microbial community.
Analysis of planted and non-planted soil samples applying PCR-DGGE technology offered evaluation of long-term patterns in fungal species richness. To evaluate the stability of DGGE patterns from different soils, comparison of planted and non-planted soil samples were compared using PCR-DGGE. The number of DNA fragments obtained from all planted soil samples by DGGE separation was far greater (14 to 15 bands) than that of the non-planted soil samples (3 to 4 bands). In addition, 14bands were observed from crop continuation soil treated with agrochemicals and 18 bands from crop rotation soil treated with microbial agents. The PCR-DGGE analysis suggests that the use of crop rotation and microbial agents benefits the fungal community more than crop continuation using agrochemicals. These results indicate that crop rotation with microbial agents was better able to support beneficial organisms, enable more effective biological control and maintain a healthier balance of nutrients,organic matter and microorganisms.
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참고문헌 (22)
참고문헌 신청
Borresen AL / 1988 / Detection of base mutations in genomic DNA using denaturing gradient gelelectrophoresis (DGGE) followed by transfer and hybridization with gene-specific
Bridge P / 2001 / Soil fungi: diversity and detection / Plant Soil 232 : 147 ~ 154
De Ley FAAM / 1997 / Functional diversity of the rhizosphere, In Plant growth-promoting rhizobacteria. Proceedings of the Fourth International Workshop on plant-growth promoting rhizobac
Eeva JV / 2000 / Direct analysis of ood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA / Mycol. Res 104 : 927 ~ 936
Esitken A / 2005 / Potential use of plant growth promoting rhizobacteria (PGPR) in organic apricot production / Proc. Int. Sci. Conf. : 90 ~ 97
Bridge P / 2001 / Soil fungi: diversity and detection / Plant Soil 232 : 147 ~ 154
De Ley FAAM / 1997 / Functional diversity of the rhizosphere, In Plant growth-promoting rhizobacteria. Proceedings of the Fourth International Workshop on plant-growth promoting rhizobac
Eeva JV / 2000 / Direct analysis of ood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA / Mycol. Res 104 : 927 ~ 936
Esitken A / 2005 / Potential use of plant growth promoting rhizobacteria (PGPR) in organic apricot production / Proc. Int. Sci. Conf. : 90 ~ 97
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