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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제4호
- 발행연도
- 2009.1
- 수록면
- 416 - 423 (8page)
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초록· 키워드
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Hepatitis B core antigen (HBcAg) is an important
serological marker used in the diagnosis of hepatitis B
virus (HBV) infections. In the current study, a fast and
efficient preparative purification protocol for truncated
HBcAg from Escherichia coli disruptate was developed.
The recombinant HBcAg was first captured by anion
exchange expanded bed adsorption chromatography
integrated with a cell disruption process. This online capture
process has shortened the process time and eliminated the
“hold-up” period that may be detrimental to the quality
of target protein. The eluted product from the expanded
bed adsorption chromatography was subsequently purified
using size-exclusion chromatography. The results showed
that this novel purification protocol achieved a recovery
yield of 45.1% with a product purity of 88.2%, which
corresponds to a purification factor of 4.5. The recovered
HBcAg is still biologically active as shown by ELISA test.
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참고문헌 (21)
참고문헌 신청
Betancourt, A. A. / 2007 / Phase I clinical trial in healthy adults of a nasal vaccine candidate containing recombinant hepatitis B surface and core antigens / Int. J. Infect
Bierau, H. / 1999 / Direct process integration of cell disruption and fluidised bed adsorption for the recovery of intracellular proteins / J. Chem. Technol. Biotech 74 : 208
Bierau, H. / 2001 / Direct process integration of cell disruption and fluidised bed adsorption in the recovery of labile microbial enzymes / Bioseparation 10 : 73 ~ 85
Bottcher, B. / 1997 / Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy / Nature 386 : 88 ~ 91
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Bierau, H. / 1999 / Direct process integration of cell disruption and fluidised bed adsorption for the recovery of intracellular proteins / J. Chem. Technol. Biotech 74 : 208
Bierau, H. / 2001 / Direct process integration of cell disruption and fluidised bed adsorption in the recovery of labile microbial enzymes / Bioseparation 10 : 73 ~ 85
Bottcher, B. / 1997 / Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy / Nature 386 : 88 ~ 91
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
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