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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제4호
- 발행연도
- 2008.1
- 수록면
- 670 - 675 (6page)
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초록· 키워드
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A laccase was isolated from the culture filtrate of the
basidiomycete Fomitella fraxinea. The enzyme was purified
to electrophoretical homogeneity using ammonium sulfate
precipitation, anion-exchange chromatography, and gelfiltration
chromatography. The enzyme was identified as
a monomeric protein with a molecular mass of 47 kDa by
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
(SDS-PAGE) and gel-filtration chromatography, and had
an isoelectric point of 3.8. The N-terminal amino acid
sequence for the enzyme was ATXSNXKTLAAD, which had
a very low similarity to the sequences previously reported
for laccases from other basidiomycetes. The optimum pH
and temperature for 2,2'-azino-bis(3-ethylbenzothiazoline-
6-sulfonate) (ABTS) were 3.0 and 70℃, respectively. The
enzyme also showed a much higher level of specific activity
for ABTS and 2,6-dimethoxyphenol (DMP), where the Km
values of the enzyme for ABTS and 2,6-DMP were 270
and 426 μM, respectively, and the Vmax values were 876
and 433.3 μM/min, respectively. The laccase activity was
completely inhibited by L-cysteine, dithiothreitol (DTT),
and sodium azide, significantly inhibited by Ni+, Mn2+,
and Ba2+, and slightly stimulated by K+ and Ca2+.
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참고문헌 (23)
참고문헌 신청
Bollag, J. M. / 1984 / Comparative studies of extracellular fungal laccases. Appl. Environ / Microbiol 48 : 849 ~ 854
Bourbonnais, R. / 1990 / Oxidation of nonphenolic substrates. An expanded role for laccase in lignin biodegradation / FEBS Lett 267 : 99 ~ 102
Bourbonnais, R. / 1995 / Lignin oxidation by laccase isozyme from Trametes versicolor and role of the mediator 2,2-azinobis(3- ethylbenzthiazoline-6-sulfonate) in kraft ligni
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Cambria, M. T. / 2000 / Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus. Protein Expr / Purif 18 : 141 ~ 147
Bourbonnais, R. / 1990 / Oxidation of nonphenolic substrates. An expanded role for laccase in lignin biodegradation / FEBS Lett 267 : 99 ~ 102
Bourbonnais, R. / 1995 / Lignin oxidation by laccase isozyme from Trametes versicolor and role of the mediator 2,2-azinobis(3- ethylbenzthiazoline-6-sulfonate) in kraft ligni
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Cambria, M. T. / 2000 / Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus. Protein Expr / Purif 18 : 141 ~ 147
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