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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제10호
- 발행연도
- 2007.1
- 수록면
- 1,607 - 1,615 (9page)
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We investigated the aplicability of the TEM-1 β-lactamase fragment complementation (BFC) system to developa strategy for the screening of protein-protein interactions inbacteria. A BFC system containing a human Fas-associated deathdomain (hFAD) and human Fas death domain (hFasDD)was generated. The hFADD-hFasD interaction was verifiedby cell survivability in ampicillin-containg medium and thecolorimetric change of nitrocefin. It was also confirmed bysteps. A coiled-coil helix coiled-coil domain-containing protein5 (CHCH5) was identified as an interacting protein of humanuracil DNA glycosylase (hUNG) from the bacterial BFC cDNAlibrary strategy. The interaction between hUNG and CHCH5 wasfurther confirmed with imunoprecipitation using a mamalianexpression system. CHCH5 enhanced the DNA glycosylaseactivity of hUNG to remove uracil from DNA duplexescontaining a U/G mismatch pair. These results sugest thatused to identify interacting protein pairs.
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참고문헌 (25)
참고문헌 신청
Campbell, R. E / 2004 / Realization of beta-lactamase as a versatile fluorogenic reporter 22 : 208 ~ 211
Drewes, G / 2003 / Global approaches to protein-protein interactions 15 : 199 ~ 205
Fields / 1989 / S. and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340 : 245 ~ 246
Galaneau, A / 2002 / Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein interactions 20 : 619 ~ 622
Ghosh, I / 2000 / Antiparallel leucine zipper-directed protein reassembly: Application to the green fluorescent protein 122 : 5658 ~ 5659
Drewes, G / 2003 / Global approaches to protein-protein interactions 15 : 199 ~ 205
Fields / 1989 / S. and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340 : 245 ~ 246
Galaneau, A / 2002 / Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein interactions 20 : 619 ~ 622
Ghosh, I / 2000 / Antiparallel leucine zipper-directed protein reassembly: Application to the green fluorescent protein 122 : 5658 ~ 5659
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