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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제12호
- 발행연도
- 2006.1
- 수록면
- 1,995 - 1,999 (5page)
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Pseudomonas sp. K82 cultured in p-hydroxybenzoateinduces protocatechuate 4,5-dioxygenase (PCD 4,5) for p-hydroxybenzoate degradation. In this study, a 6.0-kbp EcoRIfragment containing p-hydroxybenzoate degradation genes wascloned from the genome of sp. K82. Sequenceanalysis identified four genes, namely, pcaD, pcaA, pcaB, andpcaC genes known to be involved in p-hydroxybenzoatedegradation. Two putative 4-hydroxyphenylpyruvate dioxygenasesand one putative oxidoreductase were closely located by thep-hydroxybenzoate degradation genes. The gene arangementand sequences of these p-hydroxybenzoate degradation geneswere similar to those of Comamonas testosteroni andPseudomonas ochraceae. PcaAB (PCD4,5) was overexpresedin the expression vector pGEX-4T-3, purified using a GSTcolumn, and confirmed to have protocatechuate 4,5-dioxygenaseactivity. The N-terminal amino acid sequences of overexpressedPCD4,5 were identical with those of purified PCD4,5 fromPseudomonas sp. K82.
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참고문헌 (17)
참고문헌 신청
Arciero, D. M / 1990 / Protocatechuate 4,5-dioxygenase from Pseudomonas testosteroni / Methods Enzymol. 188 : 89 ~ 95
CHA CHANG-JUN / 2006 / Catechol 1,2-Dioxygenase from Rhodococcus rhodochrous N75 Capable ofMetabolizing Alkyl-Substituted Catechols / Journal of Microbiology and Biotechnolog
Chen, Y. P / 1994 / Purification and properties of a homodimeric protocatechuate 4,5-dioxygenase from Rhizobium leguminosarum / Arch. Microbiol. 161 : 191 ~ 195
Harayama, S / 1992 / Functional and evolutionary relationships among diverse oxygenases / Annu. Rev. Microbiol. 46 : 565 ~ 601
KIM, SU-JUNG / 2006 / Cloning and Expression of hpaA Gene of Korean Strain Helicobacter pyloriK51 in Oral Vaccine Delivery Vehicle Lactococcus lactis subsp. lactisMG1363 / Jo
CHA CHANG-JUN / 2006 / Catechol 1,2-Dioxygenase from Rhodococcus rhodochrous N75 Capable ofMetabolizing Alkyl-Substituted Catechols / Journal of Microbiology and Biotechnolog
Chen, Y. P / 1994 / Purification and properties of a homodimeric protocatechuate 4,5-dioxygenase from Rhizobium leguminosarum / Arch. Microbiol. 161 : 191 ~ 195
Harayama, S / 1992 / Functional and evolutionary relationships among diverse oxygenases / Annu. Rev. Microbiol. 46 : 565 ~ 601
KIM, SU-JUNG / 2006 / Cloning and Expression of hpaA Gene of Korean Strain Helicobacter pyloriK51 in Oral Vaccine Delivery Vehicle Lactococcus lactis subsp. lactisMG1363 / Jo
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