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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제12호
- 발행연도
- 2006.1
- 수록면
- 2,000 - 2,003 (4page)
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초록· 키워드
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A gene (GenBank AF096282) coding for a α- glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable α-glucosidase was produced inside of the GRAS host at 0.04 unit/mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/mg-dry cell by the inductively expressing GAL10 promoter, respectively. No α-glucosidase activities were found in the medium when the MF-alpha signal sequence from S.
cerevisiae or α-amylase signal sequence from Aspergillus oryzae were fused before the α-glucosidase gene for the secretion.
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참고문헌 (16)
참고문헌 신청
AHN,JUNGOH / 2003 / Overexpression of Thermoalkalophilic Lipase from Bacillus stearothermophilus L1 in Saccharomyces cerevisiae / Journal of Microbiology and Biotechnology 13
Bibel, M / 1998 / Isolation and analysis of genes for amylolytic enzymes of the hyperthermophilic bacterium Thermotoga maritima / FEMS Microbiol. Lett. 58 : 9 ~ 15
Bitter, G. A / 1984 / Secretion of foreign proteins from Saccharomyces cerevisiae directed by α-factor gene fusions / Proc. Natl. Acad. Sci. USA 81 : 5330 ~ 5334
Brake, A. J / 1990 / Alpha-factor leader-directed secretion of heterologous proteins from yeast / Methods Enzymol. 185 : 408 ~ 421
CHOI, JEONG JIN / 2006 / Cloning, Expression, and Characterization of a Hyperalkaline Phosphatasefrom the Thermophilic Bacterium Thermus sp. T351 / Journal of Microbiology an
Bibel, M / 1998 / Isolation and analysis of genes for amylolytic enzymes of the hyperthermophilic bacterium Thermotoga maritima / FEMS Microbiol. Lett. 58 : 9 ~ 15
Bitter, G. A / 1984 / Secretion of foreign proteins from Saccharomyces cerevisiae directed by α-factor gene fusions / Proc. Natl. Acad. Sci. USA 81 : 5330 ~ 5334
Brake, A. J / 1990 / Alpha-factor leader-directed secretion of heterologous proteins from yeast / Methods Enzymol. 185 : 408 ~ 421
CHOI, JEONG JIN / 2006 / Cloning, Expression, and Characterization of a Hyperalkaline Phosphatasefrom the Thermophilic Bacterium Thermus sp. T351 / Journal of Microbiology an
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