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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제4호
- 발행연도
- 2005.1
- 수록면
- 792 - 799 (8page)
이용수
초록· 키워드
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Two novel β-glucuronidases, which metabolize glycyrrhizin (GL) to 18β-glycyrrhetinic acid-3-O-β-D-glucuronide
(GAMG), were purified from Streptococcus LJ-22 isolated from human intestinal microflora. β-Glucuronidases I and II
were purified to apparent homogeneity, using a combination of ammonium sulfate fractionation, butyl toyopearl, QSepharose,
hydroxyapatite Ultrogel, and GL-attached Sepharose column chromatographies, with the final specific activities of 137 and 190 nmole/min/mg, respectively. The molecular sizes of both β-glucuronidases were found to be 140 kDa by gel filtration, and they consisted of two identical subunits (M.W. 67 kDa by SDS-PAGE). β-Glucuronidases I and II showed optimal activity at pH 7.0 and pH 6.5, respectively. Both purified enzymes were potently inhibited by Cu2+ and PCMS, and had maximum activity on glycyrrhizin, but did not hydrolyze p-nitrophenyl-β-glucuronides, baicalin, or GAMG. These findings suggest that the biochemical properties and substrate specificities of these enzymes are different from those of the previously purified β-glucuronidases. This is the first reported purification of sugar (not aglycone)-recognizing β- glucuronidases from intestinal bacteria.
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참고문헌 (20)
참고문헌 신청
A / 1957 / Study on the corticoid-like action of glycyrrhizin andmechanism of its action ya endocri (l) : 17 ~ 27
Bae / 2003 / Transformation of ginsenosides to compoundK by lactic acid bacteria of human intestine 13 : 9 ~ 901 14
Bell / 1978 / Purificationand properties of β-glucuronidase from human placenta : 385 ~ 391
Bradford,M / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding 72 : 248 ~ 254
Hattori / 1983 / Metabolism of glycyrrhizin by human intestinal flora : 38 ~ 42
Bae / 2003 / Transformation of ginsenosides to compoundK by lactic acid bacteria of human intestine 13 : 9 ~ 901 14
Bell / 1978 / Purificationand properties of β-glucuronidase from human placenta : 385 ~ 391
Bradford,M / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding 72 : 248 ~ 254
Hattori / 1983 / Metabolism of glycyrrhizin by human intestinal flora : 38 ~ 42
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