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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제5호
- 발행연도
- 2005.1
- 수록면
- 1,080 - 1,086 (7page)
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초록· 키워드
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Membranes prepared from Vibrio natriegensoxidized both NADH and deamino-NADH as substrates. Thewas obtained at about pH 8.5 in the presence of 0.2 M NaCl,whereas that of the NADH:ubiquinone oxidoreductase wasobtained at about pH 7.5 in the presence of 0.2 M NaCl.Electron transfer from NADH or deamino-NADH to ubiquinone-1 or oxygen generated a considerable membrane potential(ψ), which occurred even in the presence of 20Mcarbonylcyanide m-chlorophenylhydrazone (CCCP). However,the ψ was completely colapsed by the combined addition of10 M CCCP and 20M monensin. On the other hand, theactivity of the NADH oxidase and the ψ generated by theNADH oxidase system were inhibited by about 90% with10 M HQNO, whereas the activity of the NADH:ubiquinoneoxidoreductase and the ψ generated at the NADH:ubiquinoneoxidoreductase segment were inhibited by about 60%.Interestingly, the activity of the NADH:ubiquinone oxidoreductaseand the ψ generated at the NADH:ubiquinone oxidoreductasesegment were resistant to the respiratory chain inhibitors such asrotenone, capsaicin, and AgNO3, and the activity of the NADHoxidase and the ψ generated by the NADH oxidase systemwere very sensitive only to AgNO3. It was concluded, therefore,that V. natriegens cells possess a AgNO3-resistant respiratoryNa+ pump that is diferent from the AgNO3-sensitive respiratoryNa+ pump of a marine bacterium, Vibrio alginolyticus.
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참고문헌 (20)
참고문헌 신청
Asano, M / 1985 / Ag+-sensitive NADH dehydrogenase in the Na+-motive respiratory chain of the marine bacterium Vibrio alginolyticus / Agric. Biol. Chem. 49 : 2813 ~ 2817
Cho, K. H / 2000 / Enzymatic and energetic properties of the aerobic respiratory chain-linked NADH oxidase system in the marine bacterium Pseudomonas nautica / Mol. Cells 10
Dimroth, P / 1980 / A new sodium transport system energized by the decarboxylation of oxaloacetate / FEBS Lett. 122 : 234 ~ 236
Dimroth, P / 1987 / Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria / Microbiol. Rev. 51 : 320 ~ 340
Dimroth, P / 1989 / A primary respiratory Na+ pump of an anaerobic bacterium: The Na+-dependent NADH:quinone oxidoreductase of Klebsiella pneumoniae / Arch. Microbiol. 151 :
Cho, K. H / 2000 / Enzymatic and energetic properties of the aerobic respiratory chain-linked NADH oxidase system in the marine bacterium Pseudomonas nautica / Mol. Cells 10
Dimroth, P / 1980 / A new sodium transport system energized by the decarboxylation of oxaloacetate / FEBS Lett. 122 : 234 ~ 236
Dimroth, P / 1987 / Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria / Microbiol. Rev. 51 : 320 ~ 340
Dimroth, P / 1989 / A primary respiratory Na+ pump of an anaerobic bacterium: The Na+-dependent NADH:quinone oxidoreductase of Klebsiella pneumoniae / Arch. Microbiol. 151 :
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