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자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제2호
발행연도
2004.1
수록면
330 - 336 (7page)

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Fifty-two pathogenic Vibrio parahaemolyticusstrains were isolated from the environments of Busan andYeosu, Korea. Forty-three of these strains showed proteaseactivities, whereas 4 strains showed a/b hemolysin activitiesand 6 strains had urease activities. Their pathogenic factorswere not overlapping except one strain, which had bothprotease and hemolysin activities. The 6 urease-positive strains(V. parahaemolyticus YKB4, YKB14, S25, YFB20, YFO21,and YFO22) showed the same biochemical characteristics asa reference strain [V. parahaemolyticus KCTC 2471 (ureasenegative)],except for urease production. The 6 urease-positivestrains showed different urease activities in their culturesupernatant during the growth. The urease activity of S25increased sharply at the late exponential phase, and was thehighest at the initial stationary phase and was kept until thelate stationary phase. The other 5 isolates, except S25, showedurease activities at the mid-stationary phase and increasedsteadily until the late stationary phase, when the ureaseactivity was maximal. To compare the degree of virulence ofV. parahaemolyticus with different pathogenic factors, hemolysin,protease, or urease-positive strains were injected into groups of 10each of ICR mice (7- to 10-week-old males). The lethal rates ofurease-positive V. parahaemolyticus, YKB14, YKB4, and S25,were significantly high, being 50, 70, and 80%, respectively.Protease-positive V. parahaemolyticus strains FM39 and FM50showed 40% and 60% of lethal rate, respectively. HemolysinpositiveV. parahaemolyticus strains S34 and S72 had nomortality, similar to nonpathogenic V. parahaemolyticus FM12.

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