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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제2호
- 발행연도
- 2004.1
- 수록면
- 268 - 275 (8page)
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초록· 키워드
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To investigate the potential application of the singlecellgel electrophoresis (SCGE) assay to carp as an aquaticpollution monitoring technique, gill, liver, and blood cells wereisolated from carp exposed to a direct-acting mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), or indirect mutagen,benzo[a]pyrene (B[a]P), then the DNA strand breakage wasanalyzed using the assay. Based on testing 5 different cellisolation methods and 6 electrophoretic conditions, the optimizedassay conditions were found to be cell isolation by filter pressingand electrophoresis at a lower voltage and longer running time(at 0.4 V/cm for 40min). In preliminary experiments, gill andliver cells isolated from carp exposed to MNNG in vitroexhibited DNA damage signals even with 0.5 ppb exposure,which is a much higher dose than previously reported. In the gillcells isolated from carp exposed to 0.01- 0.5 ppm MNNG invivo, significant dose- and time-dependent increases wereobserved in the tail for 4 days. As such, the linear correlationbetween the relative damage index (RDI) values and time foreach dose based on the initial 48-h exposure appeared to provideeffective criteria for the genotoxicity monitoring of direct-actingmutagenic pollution. In contrast, the in vivo exposure of carp to0.25- 1.0 ppm of B[a]P for 7 days resulted in dose- and timedependentresponses in the liver cells, in which 24-h delayedresponses for metabolizing activation and gradual repair after48 h were also observed. Thus, the negative-sloped linearcorrelation between the RDI and time at each dose based onthe initial 48 h appeared to provide more effective criteria forthe genotoxicity monitoring of indirect mutagenic pollution.
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참고문헌 (28)
참고문헌 신청
A / 1991 / in liver microsomes from different fish speciesand rat : 1 ~ 13
Belpaeme / 1998 / Development and validation of the in vivo alkaline cometassay for detecting genomic damage in marine fish 415 : 167 ~ 184
Campana / 1999 / Genotoxic evaluation of the pyrethroidlambda-cyhalothrin using the micronucleus test inerythrocytes of the fish Cheirodon interruptus interruptus 438 : 155 ~
Canova / 1998 / Tissue dose, DNA adducts,oxidative DNA damage and CYP1A-immunopositiveproteins in mussels exposed to waterborne benzo[a]pyrene 399 : 17 ~ 30
De Flora / 1993 / Multiple genotoxicity biomarkers in fish exposed in situ topolluted river water viga camoira : 167 ~ 177
Belpaeme / 1998 / Development and validation of the in vivo alkaline cometassay for detecting genomic damage in marine fish 415 : 167 ~ 184
Campana / 1999 / Genotoxic evaluation of the pyrethroidlambda-cyhalothrin using the micronucleus test inerythrocytes of the fish Cheirodon interruptus interruptus 438 : 155 ~
Canova / 1998 / Tissue dose, DNA adducts,oxidative DNA damage and CYP1A-immunopositiveproteins in mussels exposed to waterborne benzo[a]pyrene 399 : 17 ~ 30
De Flora / 1993 / Multiple genotoxicity biomarkers in fish exposed in situ topolluted river water viga camoira : 167 ~ 177
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