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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제42권 제1호
발행연도
2014.1
수록면
58 - 66 (9page)

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In the course of study for a use for non-edible parts of broccoli (Brassica oleracea L), and the development of processed foodutilizing these parts, edible floret and non-edible stalk were extracted with ethanol and different organic solvent fractions wereprepared. With 10 different extracts and fractions, their useful components and various biological activities, such as antioxidant,antimicrobial and anti-proliferation activity, were investigated. The stalk has more abundant water soluble carbohydrate whencompared with the floret, and floret has higher hexane-soluble pigments. Analysis of total flavonoid and total polyphenol contentsshowed that the floret has 1.5~1.99 times higher concentrations than the stalk. Among the fractions, ethylacetate (EA)fractions have the highest amount of total flavonoid and total polyphenol. The stalk and floret possessed 9.45 and 42.01 mgtotalflavonoid/g, respectively. In the antioxidation activity assay, the EA fraction of floret showed strong radical scavengingactivity and reducing power, while the n-hexane fraction of the stalk exhibited nitrite scavenging activity. In the antimicrobialactivity assay, the EA fraction of floret showed a strong and broad-range of antibacterial activity, irrespective of gram positive orgram negative bacteria. In a while, the hexane and EA fractions revealed anti-proliferative effects against the human colorectalcancer cell HCT-116. Strong anti-proliferative activities were found in the hexane fraction of stalk (18.4% of cell viability), andthe n-butanol fraction of floret (6.9% of cell viability). Our results suggest that the further study of the characterization of activefractions and the identification of active components from different parts of broccoli are needed to develop functional foods ornovel plant-derived medicines.

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