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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 18 - 24 (7page)
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초록· 키워드
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This study was conducted to investigate optimum conditions for the production of cellulose-degrading crude enzymes by anisolated marine bacterium. A marine microorganism producing an extracellular cellulose-degrading enzyme was isolated fromthe red seaweed, Grateloupia elliptica Holmes. The isolated bacterium was identified as Cellulophaga lytica by 16S ribosomalRNA gene sequence analysis and physiological profiling and designated as Cellulophaga lytica PKA 1005. The optimum conditionsfor the growth of Cellulophaga lytica PKA 1005 were pH 7, 2% NaCl, and 30oC with 36 h incubation time. To obtain thecrude enzyme, the culture medium of the strain was centrifuged for 30 min at 12,000 × g and 4oC, and the supernatant wasused as crude enzyme. The optimum conditions for the production of the cellulose-degrading crude enzyme were pH 8, 35oC,8% carboxyl methyl cellulose, and 60 h reaction time.
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참고문헌 (22)
참고문헌 신청
Adsul MG / 2007 / Strain improvement of Penicillium janthinellum NCIM 1171 for increased cellulose production / Bioresour. Technol 98 : 1467 ~ 1473
Annamalai N / 2013 / Thermostable, haloalkaline cellulase from Bacillus halodurans CAS1 by conversion of lignocellulosic wastes / Carbohydr. Polym 94 : 409 ~ 415
Balachandrababu Malini Asha / 2012 / Purification and Characterization of a Thermophilic Cellulase from a Novel Cellulolytic Strain, Paenibacillus barcinonensis / Journal of
Johansen JE / 1999 / Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] Iytica to Cellu
Kang HJ / 2007 / Improvement of the enzymatic activity of the hyperthermophilic cellulase from Pyrococcus horikoshi / Extremophiles 11 : 251 ~ 256
Annamalai N / 2013 / Thermostable, haloalkaline cellulase from Bacillus halodurans CAS1 by conversion of lignocellulosic wastes / Carbohydr. Polym 94 : 409 ~ 415
Balachandrababu Malini Asha / 2012 / Purification and Characterization of a Thermophilic Cellulase from a Novel Cellulolytic Strain, Paenibacillus barcinonensis / Journal of
Johansen JE / 1999 / Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] Iytica to Cellu
Kang HJ / 2007 / Improvement of the enzymatic activity of the hyperthermophilic cellulase from Pyrococcus horikoshi / Extremophiles 11 : 251 ~ 256
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