지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제6호
- 발행연도
- 2014.1
- 수록면
- 854 - 861 (8page)
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초록· 키워드
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The diagnosis of Brucella abortus is mainly based on serological methods using antibody against LPS, which has diagnostic problems. Therefore, to solve this problem, we evaluated two proteins of B. abortus, Cu/Zn superoxide dismutase (SodC) and outer membrane proteins 2b porin (Omp2b). The genes were cloned and expressed in a pMAL system, and the recombinant proteins, rOmp2b and rSodC, were purified as fusion forms with maltosebinding protein. The identity of the proteins was confirmed by SDS-PAGE and Western blot analysis with sera of mice infected with B. abortus. Production of cytokines and nitric oxide (NO) was investigated in RAW 264.7 cells and mouse splenocytes after stimulation with the proteins. Moreover, cellular and humoral immune responses were investigated in BALB/c mice after immunization with the proteins. TNF-α, IL-6, and NO were significantly inducible in RAW 264.7 cells. Splenocytes of naive mice produced IFN-γ and IL-4 significantly by stimulation. Moreover, number of IgG, IFN-γ, and IL-4 producing cells were increased in immunized mice with the two proteins. Production of IgG and IgM with rOmp2b was higher than those with rSodC in immunized mice. These results suggest that the two recombinant proteins of B. abortus may be potential LPS-free proteins for diagnosis.
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참고문헌 (22)
참고문헌 신청
Al Dahouk S / 2006 / Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infection / J. Immunol. Methods 309 : 34
Baldi PC / 1996 / Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally i
Bricker BJ / 1990 / Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase / Infect. Immun 58 : 2935 ~ 2939
Cha SB / 2010 / Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus / Jpn. J. Infect. Dis 6
Cloeckaert A / 1991 / Protection conferred on mice by monoclonal antibodies directed against outer-membraneprotein antigens of Brucella / J. Med. Microbiol 34 : 175 ~ 180
Baldi PC / 1996 / Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally i
Bricker BJ / 1990 / Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase / Infect. Immun 58 : 2935 ~ 2939
Cha SB / 2010 / Comparative study of gamma interferon production in mice immunized with outer membrane proteins and whole bacteria of Brucella abortus / Jpn. J. Infect. Dis 6
Cloeckaert A / 1991 / Protection conferred on mice by monoclonal antibodies directed against outer-membraneprotein antigens of Brucella / J. Med. Microbiol 34 : 175 ~ 180
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