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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제6호
- 발행연도
- 2014.1
- 수록면
- 771 - 780 (10page)
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초록· 키워드
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A putative lipolytic enzyme gene, named as est9x, was obtained from a marine microbial metagenome of the South China Sea. Sequence analysis showed that Est9X shares lower than 27% sequence identities with the characterized lipolytic enzymes, but possesses a catalytic triad highly conserved in lipolytic enzymes of the α/β hydrolase superfamily. By phylogenetic tree construction, Est9X was grouped into a new lipase/esterase family. To understand Est9X protein in depth, it was recombinantly expressed, purified, and biochemically characterized. Within potential hydrolytic activities, only lipase/esterase activity was detected for Est9X, confirming its identity as a lipolytic enzyme. When using p-nitrophenol esters with varying lengths of fatty acid as substrates, Est9X exhibited the highest activity to the C2 substrate, indicating it is an esterase. The optimal activity of Est9X occurred at a temperature of 65°C, and Est9X was pretty stable below the optimum temperature. Distinguished from other salttolerant esterases, Est9X’s activity was tolerant to and even promoted by as high as 4 M NaCl. Our results imply that Est9X is a unique esterase and could be a potential candidate for industrial application under extreme conditions.
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참고문헌 (23)
참고문헌 신청
Arpigny JL / 1999 / Bacterial lipolytic enzymes:classification and properties / Biochem. J 343 : 177 ~ 183
Bornscheuer UT. / 2002 / Microbial carboxyl esterases: classification, properties and application in biocatalysis / FEMS Microbiol. Rev 26 : 73 ~ 81
Chen IC / 2009 / Thiruvengadam V, Hsu WH. 2009. Isolation and characterization of a novel lysine racemase from a soil metagenomic library / Appl. Environ. Microbiol 75 : 5161
Chu X / 2008 / Identification of two novel esterases from a marine metagenomic library derived from South China Sea / Appl. Microbiol. Biotechnol 80 : 615 ~ 625
Chung EJ / 2008 / Forest soil metagenome gene cluster involved in antifungal activity expression in Escherichia coli / Appl. Environ. Microbiol 74 : 723 ~ 730
Bornscheuer UT. / 2002 / Microbial carboxyl esterases: classification, properties and application in biocatalysis / FEMS Microbiol. Rev 26 : 73 ~ 81
Chen IC / 2009 / Thiruvengadam V, Hsu WH. 2009. Isolation and characterization of a novel lysine racemase from a soil metagenomic library / Appl. Environ. Microbiol 75 : 5161
Chu X / 2008 / Identification of two novel esterases from a marine metagenomic library derived from South China Sea / Appl. Microbiol. Biotechnol 80 : 615 ~ 625
Chung EJ / 2008 / Forest soil metagenome gene cluster involved in antifungal activity expression in Escherichia coli / Appl. Environ. Microbiol 74 : 723 ~ 730
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