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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제12호
- 발행연도
- 2013.1
- 수록면
- 1,757 - 1,764 (8page)
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초록· 키워드
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Trichoderma harzianum ZF-2 producing laccase was isolated from decaying samples from Shandong, China, and showed dye decolorization activities. The objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. The interactions between different fermentation parameters for laccase production were characterized using a Plackett-Burman design and the response surface methodology. The different media components were initially optimized using the conventional one-factor-at-atime method and an orthogonal test design, and a Plackett-Burman experiment was then performed to evaluate the effects on laccase production. Wheat straw powder, soybean meal,and CuSO4 were all found to have a significant influence on laccase production, and the optimal concentrations of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for laccase production were determined as follows: wheat straw powder 7.63 g/l,soybean meal 23.07 g/l, (NH4)2SO4 1 g/l, CuSO4 0.51 g/l, Tween-20 1 g/l, MgSO4 1 g/l, and KH2PO4 0.6 g/l. Using this optimized fermentation method, the yield of laccase was increased 59.68 times to 67.258 U/ml compared with the laccase production with an unoptimized medium. This is the first report on the statistical optimization of laccase production by Trichoderma harzianum ZF-2.
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참고문헌 (26)
참고문헌 신청
Abadulla E / 2000 / Decolorization and detoxication of textile dyes with a laccase from Trametes hirsuta / Appl. Environ. Microbiol 66 : 3357 ~ 3362
Assavanig A / 1992 / Isolation, characterization and function of laccase from Trichoderma / Appl. Microbiol. Biotechnol 38 : 198 ~ 202
Bollag JM / 1984 / Comparative studies of extracellular fungal laccases / Appl. Environ. Microbiol 48 : 849 ~ 854
Box GEP / 1951 / On the experimental attainment of optimum conditions / J. Roy. Stat. Soc. B 13 : 145 ~
Chen T / 2001 / A miniature biofuel cell / J. Am. Chem. Soc 123 : 8630 ~ 8631
Assavanig A / 1992 / Isolation, characterization and function of laccase from Trichoderma / Appl. Microbiol. Biotechnol 38 : 198 ~ 202
Bollag JM / 1984 / Comparative studies of extracellular fungal laccases / Appl. Environ. Microbiol 48 : 849 ~ 854
Box GEP / 1951 / On the experimental attainment of optimum conditions / J. Roy. Stat. Soc. B 13 : 145 ~
Chen T / 2001 / A miniature biofuel cell / J. Am. Chem. Soc 123 : 8630 ~ 8631
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