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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제12호
- 발행연도
- 2013.1
- 수록면
- 1,726 - 1,736 (11page)
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초록· 키워드
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Biosynthesis of indole-3-acetic acid (IAA) via the indole-3-pyruvic acid pathway involves three kinds of enzymes; aminotransferase encoded by aspC, indole-3-pyruvic acid decarboxylase encoded by ipdC, and indole-3-acetic acid dehydrogenase encoded by iad1. The ipdC from Enterobacter cloacae ATCC 13047, aspC from Escherichia coli, and iad1 from Ustilago maydis were cloned and expressed under the control of the tac and sod promoters in E. coli. According to SDS-PAGE and enzyme activity, IpdC and Iad1 showed good expression under the control of Ptac, whereas AspC was efficiently expressed by Psod originating from Corynebacterium glutamicum. The activities of IpdC, AspC, and Iad1 from the crude extracts of recombinant E.
coli Top 10 were 215.6, 5.7, and 272.1 nmol/min/mg-protein, respectively. The recombinant E.
coli DH5α expressing IpdC, AspC, and Iad1 produced about 1.1 g/l of IAA and 0.13 g/l of tryptophol (TOL) after 48 h of cultivation in LB medium with 2 g/l tryptophan. To improve IAA production, a tnaA gene mediating indole formation from tryptophan was deleted. As a result, E. coli IAA68 with expression of the three genes produced 1.8 g/l of IAA, which is a 1.6-fold increase compared with wild-type DH5α harboring the same plasmids. Moreover, the complete conversion of tryptophan to IAA was achieved by E. coli IAA68. Finally, E. coli IAA68 produced 3.0 g/l of IAA after 24 h cultivation in LB medium supplemented with 4 g/l of tryptophan.
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참고문헌 (19)
참고문헌 신청
Apine OA / 2011 / Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM / J. Appl. Microbiol 110 : 1235 ~ 1244
Atsumi S / 2010 / Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/ alcohol dehydrogenase genes / Appl. Microbiol
Basse CW / 1996 / Two potential indole-3-acetaldehyde dehydrogenases in the phytopathogenic fungus Ustilago maydis / Eur. J. Biochem 242 : 648 ~ 656
Baudoin E / 2010 / Effects of Azospirillum brasilense with genetically modified auxin biosynthesis gene ipdC upon the diversity of the indigenous microbiota of the wheat rhiz
Becker J / 2005 / Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine pr
Atsumi S / 2010 / Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/ alcohol dehydrogenase genes / Appl. Microbiol
Basse CW / 1996 / Two potential indole-3-acetaldehyde dehydrogenases in the phytopathogenic fungus Ustilago maydis / Eur. J. Biochem 242 : 648 ~ 656
Baudoin E / 2010 / Effects of Azospirillum brasilense with genetically modified auxin biosynthesis gene ipdC upon the diversity of the indigenous microbiota of the wheat rhiz
Becker J / 2005 / Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine pr
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