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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제12호
- 발행연도
- 2013.1
- 수록면
- 1,708 - 1,716 (9page)
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초록· 키워드
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Conventional molecular detection methods cannot distinguish between viable and dead Escherichia coli O157 cells. In this study, the loop-mediated isothermal amplification (LAMP)method combined with propidium monoazide (PMA) treatment was developed to selectively detect viable E. coli O157 cells. Four primers, including outer primers and inner primers, were specially designed for the recognition of six distinct sequences on the serogroups (O157) of the specific rfbE gene of the E. coli O157 genome. PMA selectively penetrated through the compromised cell membranes and intercalated into DNA. Amplification of DNA from dead cells was completely inhibited by 3.0 μg/ml PMA, whereas the DNA derived from viable cells was amplified remarkably within 1 h by PMA-LAMP. Exhibiting high sensitivity and specificity, PMA-LAMP is a suitable method for evaluating the inactivation efficacy of slightly acidic electrolyzed water in broth. PMA-LAMP can selectively detect viable E. coli O157 cells.
This study offers a novel molecular detection method to distinguish between viable and dead E. coli O157 cells.
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참고문헌 (20)
참고문헌 신청
Asiello PJ / 2011 / Miniaturized isothermal nucleic acid amplification, a review / Lab. Chip 11 : 1420 ~ 1430
Bilge SS / 1996 / Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus / Infect. Immun 64 : 4795 ~ 4801
Chang B / 2010 / Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells / Jap. J. Infect. Diseases 63 : 119 ~ 123
Chassagne L / 2009 / Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction / Diagn. Microbiol
Chen SY / 2011 / Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification / Appl. Environ. Microbiol 77 :
Bilge SS / 1996 / Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus / Infect. Immun 64 : 4795 ~ 4801
Chang B / 2010 / Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells / Jap. J. Infect. Diseases 63 : 119 ~ 123
Chassagne L / 2009 / Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction / Diagn. Microbiol
Chen SY / 2011 / Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification / Appl. Environ. Microbiol 77 :
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