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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제12호
- 발행연도
- 2013.1
- 수록면
- 1,747 - 1,756 (10page)
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The aim of this paper was to study the effect of spent medium recycle on Spodoptera exigua Se301 cell line proliferation, metabolism, and baculovirus production when grown in batch suspension cultures in Ex-Cell 420 serum-free medium. The results showed that the recycle of 20% of spent medium from a culture in mid-exponential growth phase improved growth relative to a control culture grown in fresh medium. Although both glucose and glutamine were still present at the end of the growth phase, glutamate was always completely exhausted.
The pattern of the specific glucose and lactate consumption and production rates, as well as the specific glutamine and glutamate consumption rates, suggests a metabolic shift at spent medium recycle values of over 60%, with a decrease in the efficiency of glucose utilization and an increase in glutamate consumption to fuel energy metabolism. Baculovirus infection provoked a change in the metabolic pattern of Se301 cells, although a beneficial effect of spent medium recycle was also observed. Both growth rate and maximum viable cell density decreased relative to uninfected cultures. The efficiency of glucose utilization was dramatically reduced in those cultures containing the lowest percentages of spent medium,whereas glutamine and glutamate consumption was modulated, thereby suggesting that infected cells were devoted to virus replication, retaining their ability to incorporate the nutrients required to support viral replication. Recycle of 20% of spent medium increased baculovirus production by around 90%, thus showing the link between cell growth and baculovirus production.
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참고문헌 (22)
참고문헌 신청
Airenne KJ / 2013 / Baculovirus: an insect-derived vector for diverse gene transfer applications / Mol. Ther 21 : 739 ~ 749
Andersen A / 2000 / A new secreted insect protein belonging to the immunoglobulin superfamily binds insulin and related peptides and inhibits their activities / J. Biol. Chem
Beas-Catena A / 2011 / Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovirus (SeMNPV) / Cyt
Beas-Catena A / 2013 / Adaptation of the Spodoptera exigua Se301 insect cell line to grow in serumfree suspended culture. Comparison of SeMNPV productivity in serum-free and
Bedard C / 1993 / Growth, nutrient consumption, and end-product accumulation in Sf-9 and BTI-EAA insect cell cultures: insights into growth limitation and metabolism / Biotec
Andersen A / 2000 / A new secreted insect protein belonging to the immunoglobulin superfamily binds insulin and related peptides and inhibits their activities / J. Biol. Chem
Beas-Catena A / 2011 / Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovirus (SeMNPV) / Cyt
Beas-Catena A / 2013 / Adaptation of the Spodoptera exigua Se301 insect cell line to grow in serumfree suspended culture. Comparison of SeMNPV productivity in serum-free and
Bedard C / 1993 / Growth, nutrient consumption, and end-product accumulation in Sf-9 and BTI-EAA insect cell cultures: insights into growth limitation and metabolism / Biotec
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