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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제12호
- 발행연도
- 2013.1
- 수록면
- 1,664 - 1,672 (9page)
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In Salmonella enterica serovar Typhimurium, many genes encoded within Salmonella pathogenicity island 1 (SPI1) are required to induce intestinal/diarrheal disease. In this study,we compared the expression of four SPI1 genes (hilA, invF, prgH, and sipC) under shaking and standing culture conditions and found that the expression of these genes was highest during the transition from the exponential to stationary phase under shaking conditions. To identify regulators associated with the stationary phase-dependent activation of SPI1, the effects of selected regulatory genes, including relA/spoT (ppGpp), luxS, ihfB, hfq, and arcA, on the expression of hilA and invF were compared under shaking conditions. Mutations in the hfq and arcA genes caused a reduction in hilA and invF expression (more than 2-fold) in the early stationary phase only, whereas the lack of ppGpp and IHF decreased hilA and invF gene expression during the entire stationary phase. We also found that hfq and arcA mutations caused a reduction of hilD expression upon entry into the stationary phase under shaking culture conditions. Taken together, these results suggest that Hfq and ArcA regulate the hilD promoter, causing an accumulation of HilD, which can trigger a stationary phase-dependent activation of SPI1 genes under shaking culture conditions.
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참고문헌 (22)
참고문헌 신청
Ackermann M / 2008 / Self-destructive cooperation mediated by phenotypic noise / Nature 454 : 987 ~ 990
Ansong C / 2009 / Global systems-level analysis of Hfq and SmpB deletion mutants in Salmonella: implications for virulence and global protein translation / PLoS One 4 : e4809
Bajaj V / 1996 / Co-ordinate regulation of Salmonella Typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression / Mol. Micr
Baxter MA / 2003 / HilE interacts with HilD and negatively regulates hilA transcription and expression of the Salmonella enterica serovar Typhimurium invasive phenotype / Inf
Boddicker JD / 2003 / Transcription of the Salmonella invasion gene activator, hilA, requires HilD activation in the absence of negative regulators / J. Bacteriol 185 : 525 ~
Ansong C / 2009 / Global systems-level analysis of Hfq and SmpB deletion mutants in Salmonella: implications for virulence and global protein translation / PLoS One 4 : e4809
Bajaj V / 1996 / Co-ordinate regulation of Salmonella Typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression / Mol. Micr
Baxter MA / 2003 / HilE interacts with HilD and negatively regulates hilA transcription and expression of the Salmonella enterica serovar Typhimurium invasive phenotype / Inf
Boddicker JD / 2003 / Transcription of the Salmonella invasion gene activator, hilA, requires HilD activation in the absence of negative regulators / J. Bacteriol 185 : 525 ~
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