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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제7호
- 발행연도
- 2013.1
- 수록면
- 984 - 992 (9page)
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The entire nucleotide sequence of the xylose reductase (XR) gene in Candida milleri CBS8195sourdough yeast was determined by degenerate polymerase chain reaction (PCR) and genome walking. The sequence analysis revealed an open-reading frame of 981 bp that encoded 326amino acids with a predicted molecular mass of 36.7 kDa. The deduced amino acid sequence of XR of C. milleri was 64.7% homologous to that of Kluyveromyces lactis. The cloned XR gene was expressed in Saccharomyces cerevisiae, and the resulting recombinant S. cerevisiae strain produced xylitol from xylose, indicating that the C. milleri XR introduced into S. cerevisiae is functional. An enzymatic activity assay and semiquantitative reverse transcription-PCR revealed that the expression of CmXR was induced by xylose. The GenBank Accession No. for CmXR is KC599203.
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참고문헌 (23)
참고문헌 신청
[학
Akinterinwa O / 2008 / Metabolic engineering for bioproduction of sugar alcohols / Curr. Opin. Biotechnol 19 : 461 ~ 467
Amore R / 1991 / Cloning and expression in Saccharomyces cerevisiae of the NAD(P)H-dependent xylose reductase-encoding gene (XYL1) from the xylose-assimilating yeast Pichia s
Belazzi T / 1991 / Negative regulation of transcription of the Saccharomyces cerevisiae catalase T (CTT1) gene by cAMP is mediated by a positive control element / EMBO J 10 :
Bengtsson O / 2009 / Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae / B
Akinterinwa O / 2008 / Metabolic engineering for bioproduction of sugar alcohols / Curr. Opin. Biotechnol 19 : 461 ~ 467
Amore R / 1991 / Cloning and expression in Saccharomyces cerevisiae of the NAD(P)H-dependent xylose reductase-encoding gene (XYL1) from the xylose-assimilating yeast Pichia s
Belazzi T / 1991 / Negative regulation of transcription of the Saccharomyces cerevisiae catalase T (CTT1) gene by cAMP is mediated by a positive control element / EMBO J 10 :
Bengtsson O / 2009 / Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae / B
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