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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제7호
- 발행연도
- 2013.1
- 수록면
- 993 - 1,003 (11page)
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Methanotrophs are the most important sink of CH4, which is a more highly potent greenhouse gas than CO2. Methanotrophic abundance and community diversity in cover soils from two typical semi-aerobic landfills (SALs) in China were detected using real-time polymerase chain reaction (real-time-PCR) and denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA genes, respectively. Real time-PCR showed that Type I methanotrophs ranged from 1.07× 106 to 2.34 × 107 copies/g soil and that of Type II methanotrophs from 1.51 × 107 to 1.83 × 108copies/g soil. The ratio of Type II to Type I methanotrophic copy numbers ranged from 5.61 to 21.89, indicating that Type II methanotrophs dominated in SAL. DGGE revealed that Type I methanotrophs responded more sensitively to the environment, changing as the community structure varied with different soil types and locations. Methylobacter, Methylosarcina, and Methylomicrobium for Type I, and Methylocystis for Type II were most prevalent in the SAL cover layer. Abundant interflow O2 with high CH4 concentration in SALs is the reason for the higher population density of methanotrophs and the higher enrichment of Type II methanotrophs compared with anaerobic landfills and other ecosystems, which proved a conclusion that increasing the oxygen supply in a landfill cover layer would greatly improve CH4 mitigation.
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참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)] -
Ait-Benichou S / 2009 / Methanotrophs and methanotrophic activity in engineered landfill biocovers / Waste Manag 29 : 2509 ~ 2517
Amaral JA / 1995 / Growth of methanotrophs in methane and oxygen counter gradients / FEMS Microbiol. Lett 126 : 215 ~ 220
Auman AJ / 2000 / Molecular characterization of methanotrophic isolates from freshwater lake sediment / Appl. Environ. Microbiol 66 : 5259 ~ 5266
Aymerich T / 2003 / Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic staphylococci from artisanal low-acid sausages / Appl. Environ.
Ait-Benichou S / 2009 / Methanotrophs and methanotrophic activity in engineered landfill biocovers / Waste Manag 29 : 2509 ~ 2517
Amaral JA / 1995 / Growth of methanotrophs in methane and oxygen counter gradients / FEMS Microbiol. Lett 126 : 215 ~ 220
Auman AJ / 2000 / Molecular characterization of methanotrophic isolates from freshwater lake sediment / Appl. Environ. Microbiol 66 : 5259 ~ 5266
Aymerich T / 2003 / Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic staphylococci from artisanal low-acid sausages / Appl. Environ.
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