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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제8호
- 발행연도
- 2012.1
- 수록면
- 1,059 - 1,065 (7page)
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초록· 키워드
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Biocatalytic transfer of oxygen in isolated cytochrome P450 or whole microbial cells is an elegant and efficient way to achieve selective hydroxylation. Cytochrome P450CYP105P2 was isolated from Streptomyces peucetius that showed a high degree of amino acid identity with hydroxylases. Previously performed homology modeling,and subsequent docking of the model with flavone,displayed a reasonable docked structure. Therefore, in this study, in a pursuit to hydroxylate the flavone ring,CYP105P2 was co-expressed in a two-vector system with putidaredoxin reductase (camA) and putidaredoxin (camB)from Pseudomonas putida for efficient electron transport.
HPLC analysis of the isolated product, together with LCMS analysis, showed a monohydroxylated flavone, which was further established by subsequent ESI/MS-MS. A successful 10.35% yield was achieved with the whole-cell bioconversion reaction in Escherichia coli. We verified that CYP105P2 is a potential bacterial hydroxylase.
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참고문헌 (23)
참고문헌 신청
Boue, M. S. / 2003 / Identification of flavone aglycones and glycosides in soybean pods by liquid chromatography-tandem mass spectrometry / J. Chromatogr. A 991 : 61 ~ 68
Canivenc-Lavier, M. C. / 1996 / Differential effects of nonhydroxylated flavonoids as inducers of cytochrome P450 1A and 2B isozymes in rat liver / Toxicol. Appl. Pharmacol 1
Chen, L. J. / 2003 / Separation and identification of flavonoids in an extract from the seeds of Oroxylum indicum by CCC / J. Liq. Chromatogr. Relat. Technol 26 : 1623 ~ 1636
Ciolino, H. P. / 1998 / Diosmin and diosmetin are agonists of the aryl hydrocarbon receptor that differentially affect cytochrome P450 1A1 activity / Cancer Res 58 : 2754 ~ 2
Das, S. / 2006 / Microbial and enzymatic transformations of flavonoids / J. Nat. Prod 69 : 499 ~ 508
Canivenc-Lavier, M. C. / 1996 / Differential effects of nonhydroxylated flavonoids as inducers of cytochrome P450 1A and 2B isozymes in rat liver / Toxicol. Appl. Pharmacol 1
Chen, L. J. / 2003 / Separation and identification of flavonoids in an extract from the seeds of Oroxylum indicum by CCC / J. Liq. Chromatogr. Relat. Technol 26 : 1623 ~ 1636
Ciolino, H. P. / 1998 / Diosmin and diosmetin are agonists of the aryl hydrocarbon receptor that differentially affect cytochrome P450 1A1 activity / Cancer Res 58 : 2754 ~ 2
Das, S. / 2006 / Microbial and enzymatic transformations of flavonoids / J. Nat. Prod 69 : 499 ~ 508
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