지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제1호
- 발행연도
- 2012.1
- 수록면
- 92 - 99 (8page)
이용수
초록· 키워드
오류제보하기
The optimal physical factors affecting enzyme production in an airlift fermenter have not been studied so far.
Therefore, the physical parameters such as aeration rate,pH, and temperature affecting PLA-degrading enzyme production by Actinomadura keratinilytica strain T16-1 in a 3 l airlift fermenter were investigated. The response surface methodology (RSM) was used to optimize PLAdegrading enzyme production by implementing the central composite design. The optimal conditions for higher production of PLA-degrading enzyme were aeration rate of 0.43 vvm, pH of 6.85, and temperature at 46oC. Under these conditions, the model predicted a PLA-degrading activity of 254 U/ml. Verification of the optimization showed that PLA-degrading enzyme production of 257 U/ml was observed after 3 days cultivation under the optimal conditions in a 3 l airlift fermenter. The production under the optimized condition in the airlift fermenter was higher than un-optimized condition by 1.7 folds and 12 folds with un-optimized medium or condition in shake flasks. This is the first report on the optimization of environmental conditions for improvement of PLA-degrading enzyme production in a 3 l airlift fermenter by using a statistical analysis method. Moreover, the crude PLA-degrading enzyme could be adsorbed to the substrate and degraded PLA powder to produce lactic acid as degradation products. Therefore, this incident indicates that PLAdegrading enzyme produced by Actinomadura keratinilytica NBRC 104111 strain T16-1 has a potential to degrade PLA to lactic acid as a monomer and can be used for the recycle of PLA polymer.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Anbu, P. / 2007 / Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis / Bioresour. Technol 98 : 1298 ~ 1303
Box, G. E. P. / 1951 / On the experimental attainment of optimum conditions / J. Roy. Stat. Soc. B 13 : 1 ~ 45
Jarerat, A. / 2001 / Degradation of poly(L-lactide) by a fungus / Macromol. Biosci 1 : 136 ~ 140
Jarerat, A. / 2004 / Microbial poly(Llactide)- degrading enzyme induced by amino acids, peptides, and poly(L-amino acids) / J. Polym. Eviron 12 : 139 ~ 146
Jarerat, A. / 2006 / Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide) / Appl. Microbiol. Biotechnol 72 :
Box, G. E. P. / 1951 / On the experimental attainment of optimum conditions / J. Roy. Stat. Soc. B 13 : 1 ~ 45
Jarerat, A. / 2001 / Degradation of poly(L-lactide) by a fungus / Macromol. Biosci 1 : 136 ~ 140
Jarerat, A. / 2004 / Microbial poly(Llactide)- degrading enzyme induced by amino acids, peptides, and poly(L-amino acids) / J. Polym. Eviron 12 : 139 ~ 146
Jarerat, A. / 2006 / Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide) / Appl. Microbiol. Biotechnol 72 :
이 논문의 저자 정보
최근 본 자료
댓글(0)
0