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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제1호
- 발행연도
- 2012.1
- 수록면
- 69 - 79 (11page)
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초록· 키워드
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In an ongoing survey of the bioactive potential of microorganisms from Ladakh, India, the culture medium of a bacterial strain of a new Pseudomonas sp., strain ICTB-745, isolated from an alkaline soil sample collected from Leh, Ladakh, India, was found to contain metabolites that exhibited broad-spectrum antimicrobial and biosurfactant activities. Bioactivity-guided purification resulted in the isolation of four bioactive compounds.
Their chemical structures were elucidated by 1H and 13C NMR, 2D-NMR (HMBC, HSQC, 1H,1H-COSY, and DEPT-135), FT-IR, and mass spectroscopic methods, and were identified as 1-hydroxyphenazine, phenazine-1-carboxylic acid (PCA), rhamnolipid-1 (RL-1), and rhamnolipid-2(RL-2). These metabolites exhibited various biological activities like antimicrobial and efficient cytotoxic potencies against different human tumor cell lines such as HeLa,HepG2, A549, and MDA MB 231. RL-1 and RL-2 exhibited a dose-dependent antifeedant activity against Spodoptera litura, producing about 82.06% and 73.66% antifeedant activity, whereas PCA showed a moderate antifeedant activity (63.67%) at 60 μg/cm2 area of castor leaf. Furthermore,PCA, RL-1, and RL-2 exhibited about 65%, 52%, and 47% mortality, respectively, against Rhyzopertha dominica at 20 μg/ml. This is the first report of rhamnolipids as antifeedant metabolites against Spodoptera litura and as insecticidal metabolites against Rhyzopertha dominica.
The metabolites from Pseudomonas sp. strain ICTB-745have interesting potential for use as a biopesticide in pest control programs.
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참고문헌 (23)
참고문헌 신청
Abalos, A. / 2001 / Physicochemical and antimicrobial properties of new rhamnolipids by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes / Langmuir 17 : 1367 ~ 13
Abbott, W. S. / 1925 / A method of computing the effectiveness of an insecticide / J. Econ. Entomol 18 : 265 ~ 267
Abdel-Mawgoud, A. M. / 2010 / Rhamnolipids: Diversity of structures, microbial origins and roles / Appl. Microbiol. Biotechnol 86 : 1323 ~ 1336
Abivardi, C. / 1984 / Tests with extracts of 21 medicinal plants for anti-feedant activity against larvae of Pteris brassicae L. (Lepteridae) / Bull. Soc. Entomol. Swisse 57
Bernays, E. A. / 2000 / Taste sensitivity of insect herbivores to deterrents is greater in specialists than in generalists: A behavioural test of the hypothesis with two clos
Abbott, W. S. / 1925 / A method of computing the effectiveness of an insecticide / J. Econ. Entomol 18 : 265 ~ 267
Abdel-Mawgoud, A. M. / 2010 / Rhamnolipids: Diversity of structures, microbial origins and roles / Appl. Microbiol. Biotechnol 86 : 1323 ~ 1336
Abivardi, C. / 1984 / Tests with extracts of 21 medicinal plants for anti-feedant activity against larvae of Pteris brassicae L. (Lepteridae) / Bull. Soc. Entomol. Swisse 57
Bernays, E. A. / 2000 / Taste sensitivity of insect herbivores to deterrents is greater in specialists than in generalists: A behavioural test of the hypothesis with two clos
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