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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제1호
- 발행연도
- 2012.1
- 수록면
- 84 - 91 (8page)
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Two staphylococcal lipases were obtained from Staphylococcus epidermidis S2 and Staphylococcus aureus S11 isolated from sebaceous areas on the skin of the human face. The molecular mass of both enzymes was estimated to be 45 kDa by SDS-PAGE. S2 lipase displayed its highest activity in the hydrolysis of olive oil at 32oC and pH 8,whereas S11 lipase showed optimal activity at 31oC and pH 8.5. The S2 lipase showed the property of cold-adaptation,with activation energy of 6.52 kcal/mol. In contrast, S11lipase’s activation energy, at 21 kcal/mol, was more characteristic of mesophilic lipases. S2 lipase was stable up to 45oC and within the pH range from 5 to 9, whereas S11 lipase was stable up to 50oC and from pH 6 to 10. Both enzymes had high activity against tributyrin, waste soybean oil, and fish oil. Sequence analysis of the S2 lipase gene showed an open reading frame of 2,067 bp encoding a signal peptide (35 aa), a pro-peptide (267 aa), and a mature enzyme (386 aa); the S11 lipase gene, at 2,076 bp,also encoded a signal peptide (37 aa), pro-peptide (255 aa),and mature enzyme (399 aa). The two enzymes maintained amino acid sequence identity of 98-99% with other similar staphylococcal lipases. Their microbial origins and biochemical properties may make these staphylococcal lipases isolated from facial sebaceous skin suitable for use as catalysts in the cosmetic, medicinal, food, or detergent industries.
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참고문헌 (24)
참고문헌 신청
Akoh, C. C. / 2007 / Enzymatic approach to biodiesel production / J. Agric. Food Chem 55 : 8995 ~ 9005
Arpigny, J. L. / 1997 / Molecular adaptation to cold of an Antarctic bacterial lipase / J. Mol. Catal. B Enzym 3 : 29 ~ 35
Choo, D. W. / 1997 / A cold-adapted lipase of an Alaskan psychrotroph,Pseudomonas sp. strain B11-1: Gene cloning and enzymepurification and characterization / Appl. Environ.
Christensson, B. / 1985 / A new serological assay for Staphylococcus aureus infections: Detection of IgG antibodies to S. aureus lipase with an enzymelinked immunosorbent ass
Farrel, A. M. / 1993 / Molecular analysis and expression of the lipase of Staphylococcus epidermidis / J. Gen. Microbiol 139 : 267 ~ 277
Arpigny, J. L. / 1997 / Molecular adaptation to cold of an Antarctic bacterial lipase / J. Mol. Catal. B Enzym 3 : 29 ~ 35
Choo, D. W. / 1997 / A cold-adapted lipase of an Alaskan psychrotroph,Pseudomonas sp. strain B11-1: Gene cloning and enzymepurification and characterization / Appl. Environ.
Christensson, B. / 1985 / A new serological assay for Staphylococcus aureus infections: Detection of IgG antibodies to S. aureus lipase with an enzymelinked immunosorbent ass
Farrel, A. M. / 1993 / Molecular analysis and expression of the lipase of Staphylococcus epidermidis / J. Gen. Microbiol 139 : 267 ~ 277
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