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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제11호
- 발행연도
- 2011.1
- 수록면
- 1,116 - 1,122 (7page)
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In this study, site-directed mutagenesis was performed on the β-agarase AgaA gene from Zobellia galactanivorans to improve its catalytic activity and thermostability. The activities of three mutant enzymes, S63K, C253I, and S63K-C253I, were 126% (1,757.78 U/mg), 2.4% (33.47 U/mg),and 0.57% (8.01 U/mg), respectively, relative to the wildtype β-agarase AgaA (1,392.61 U/mg) at 40℃. The stability of the mutant S63K enzyme was 125% of the wild-type up to 45℃, where agar is in a sol state. The mutant S63K enzyme produced 166%, 257%, and 220% more neoagarohexaose, and 230%, 427%, and 350% more neoagarotetraose than the wild-type in sol, gel, and nonmelted powder agar, respectively, at 45℃ over 24 h. The mutant S63K enzyme produced 50% more neoagarooligosaccharides from agar than the wild-type β-agarase AgaA from agarose under the same conditions. Thus, mutant S63K β-agarase AgaA may be useful for the production of functional neoagarooligosaccharides.
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참고문헌 (21)
참고문헌 신청
Allouch, J / 2003 / The three-dimensional structures of two β-agarases / J. Biol. Chem 278 : 47171 ~ 47180
Allouch, J. / 2004 / Parallel substrate binding sites in a β-agarase suggest a novel mode of action on double-helical agarose / Structure 12 : 623 ~ 632
Araki, C / 1959 / Seaweed polysaccharides in Carbohydrate Chemistry of Substances of Biological Interest / Proceedings of the 4th International Congress of Biochemistry : 15 ~ 30
Bannikova, G. E. / 2008 / The thermophilic bacteria hydrolyzing agar: Characterization of thermostable agarase / J. Biol. Chem. Microbiol 44 : 366 ~ 369
Cao, L / 2005 / Immobilised enzymes: Science or art / Curr. Opin. Chem. Biol 9 : 217 ~ 226
Allouch, J. / 2004 / Parallel substrate binding sites in a β-agarase suggest a novel mode of action on double-helical agarose / Structure 12 : 623 ~ 632
Araki, C / 1959 / Seaweed polysaccharides in Carbohydrate Chemistry of Substances of Biological Interest / Proceedings of the 4th International Congress of Biochemistry : 15 ~ 30
Bannikova, G. E. / 2008 / The thermophilic bacteria hydrolyzing agar: Characterization of thermostable agarase / J. Biol. Chem. Microbiol 44 : 366 ~ 369
Cao, L / 2005 / Immobilised enzymes: Science or art / Curr. Opin. Chem. Biol 9 : 217 ~ 226
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