지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제4호
- 발행연도
- 2011.1
- 수록면
- 421 - 429 (9page)
이용수
초록· 키워드
오류제보하기
For precise identification of a Lactobacillus K1 isolate,LC-MS/MS analysis of the putative surface layer protein was performed. The results obtained from LTQ-FT-ICR mass spectrometry confirmed that the analyzed protein spot is the surface layer protein originating from Lb. helveticus species. Moreover, the identified protein has the highest similarity with the surface layer protein from Lb. helveticus R0052. To evaluate the proteomic study, multilocus sequence analysis of selected housekeeping gene sequences was performed. Combination of 16S rRNA sequencing with partial sequences for the genes encoding the RNA polymerase alpha subunit (rpoA), phenylalanyl-tRNA synthase alpha subunit (pheS), translational elongation factor Tu (tuf), and Hsp60 chaperonins (groEL) also allowed to classify the analyzed isolate as Lb. helveticus. Further classification at the strain level was achieved by sequencing of the slp gene. This gene showed 99.8% identity with the corresponding slp gene of Lb. helveticus R0052, which is in good agreement with data obtained by nano-HPLC coupled to an LTQ-FT-ICR mass spectrometer. Finally, LC-MS/MS analysis of surface layer proteins extracted from three other Lactobacillus strains proved that the proposed method is the appropriate molecular tool for the identification of S-layer-possessing lactobacilli at the species and even strain levels.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Anderson, J. W / 1999 / Effect of fermented milk (yogurt) containing Lactobacillus acidophilus L1 on serum cholesterol in hypercholesterolemic humans / J. Am. Coll. Nutr 18 :
Antikainen, J / 2002 / Domains in the S-layer protein CbsA of Lactobacillus cispatus involved in adherence to collagens, laminin and lipoteichoic acids and in self-assembly /
Avall-Jaaskelainen, S / 2005 / Lactobacillus surface layers and their applications / FEMS Microbiol. Rev 29 : 511 ~ 529
Avall-Jaaskelainen, S / 2008 / Identification and characterization of domains responsible for self-assembly and cell wall binding of the surface layer protein of Lactobacillu
Axelsson, L / 2004 / Lactic acid bacteria: Classification and physiology in Microbiological and Functional Aspects, 3rd Ed / Marcel Dekker : 1 ~ 66
Antikainen, J / 2002 / Domains in the S-layer protein CbsA of Lactobacillus cispatus involved in adherence to collagens, laminin and lipoteichoic acids and in self-assembly /
Avall-Jaaskelainen, S / 2005 / Lactobacillus surface layers and their applications / FEMS Microbiol. Rev 29 : 511 ~ 529
Avall-Jaaskelainen, S / 2008 / Identification and characterization of domains responsible for self-assembly and cell wall binding of the surface layer protein of Lactobacillu
Axelsson, L / 2004 / Lactic acid bacteria: Classification and physiology in Microbiological and Functional Aspects, 3rd Ed / Marcel Dekker : 1 ~ 66
이 논문의 저자 정보
최근 본 자료
댓글(0)
0