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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 70 - 76 (7page)
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The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.
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참고문헌 (23)
참고문헌 신청
Ahn, J. D / 2002 / Inhibitory effects of novel AP-1 decoy oligodeoxynucleotides on vascular smooth muscle cell proliferation in vitro and neointimal formation in vivo / Circ
Asyali, M. H / 2004 / Assessment of reliability of microarray data and estimation of signal thresholds using mixture modeling / leic Acids Res. 32 : 2323 ~ 2335
Busto, R / 2003 / VIP and PACAP receptors coupled to adenylyl cyclase in human lung cancer: a study in biopsy specimens / Peptides 24 : 429 ~ 436
Chang, C. C / 2004 / Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis / J. Natl. Cancer Inst. 96 : 364 ~ 375
DeRisi, J. L / 1997 / Exploring the metabolic and genetic control of gene expression on a genomic scale / Science 278 : 680 ~ 686
Asyali, M. H / 2004 / Assessment of reliability of microarray data and estimation of signal thresholds using mixture modeling / leic Acids Res. 32 : 2323 ~ 2335
Busto, R / 2003 / VIP and PACAP receptors coupled to adenylyl cyclase in human lung cancer: a study in biopsy specimens / Peptides 24 : 429 ~ 436
Chang, C. C / 2004 / Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis / J. Natl. Cancer Inst. 96 : 364 ~ 375
DeRisi, J. L / 1997 / Exploring the metabolic and genetic control of gene expression on a genomic scale / Science 278 : 680 ~ 686
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