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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제5호
- 발행연도
- 2018.1
- 수록면
- 821 - 825 (5page)
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초록· 키워드
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A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and a 184-kb split-YAC. The 44-kb split-mini YAC was amplified a maximum of 9-fold, and the activity of the reporter enzymes integrated into the split-mini YAC increased about 5-7-fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number.
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등록된 정보가 없습니다.
참고문헌 (11)
참고문헌 신청
Burke D / 1987 / Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vector / Science 236:806~811
Pavan WJ / 1991 / High-efficiency yeast artificial chromosome fragmentation vectors / Gene 106:125~127
Emanuel SL / 1995 / New vectors for manipulation and selection of functional yeast artificial chromosomes (YACs) containing human DNA inserts / Gene 155:167~174
Kim YH / 2005 / A yeast artificial chromosome-splitting vector designed for precise manipulation of specific plant chromosome region / J. Biosci. Bioeng. 99:55~60
Smith DR / 1990 / Amplification of large artificial chromosomes / Proc. Natl. Acad. Sci. USA 87:8242~8246
Pavan WJ / 1991 / High-efficiency yeast artificial chromosome fragmentation vectors / Gene 106:125~127
Emanuel SL / 1995 / New vectors for manipulation and selection of functional yeast artificial chromosomes (YACs) containing human DNA inserts / Gene 155:167~174
Kim YH / 2005 / A yeast artificial chromosome-splitting vector designed for precise manipulation of specific plant chromosome region / J. Biosci. Bioeng. 99:55~60
Smith DR / 1990 / Amplification of large artificial chromosomes / Proc. Natl. Acad. Sci. USA 87:8242~8246
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