지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제8호
- 발행연도
- 2017.1
- 수록면
- 1,519 - 1,528 (10page)
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초록· 키워드
오류제보하기
Foodborne contamination and salmonellosis caused by Salmonella Enteritidis (S. Enteritidis) are a significant threat to human health and poultry enterprises. Outer membrane vesicles (OMVs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (LPS), outer membrane proteins (OMPs), and phospholipids, as well as periplasmic components. In the present study, we purified OMVs derived from S. Enteritidis and analyzed their characteristics through silver staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis. In total, 108 proteins were identified in S. Enteritidis OMVs through liquid chromatography tandem mass spectrometry analysis, and OMPs, periplasmic proteins, and extracellular proteins (49.9% of total proteins) were found to be enriched in the OMVs compared with bacterial cells. Furthermore, native OMVs used in immunizations by either the intranasal route or the intraperitoneal route could elicit significant humoral and mucosal immune responses and provide strong protective efficiency against a lethal dose (~100-fold LD50) of the wild-type S. Enteritidis infection. These results indicated that S. Enteritidis OMVs might be an ideal vaccine strategy for preventing S. Enteritidis diseases.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bomberger JM / 2009 / Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa o u ter membrane vesicles / PLoS Pathog 5 : e1000382 ~
Bonnington KE / 2014 / Protein selection and export via outer membrane vesicles / Biochim. Biophys. Acta 1843 : 1612 ~ 1619
Chai SJ / 2012 / Salmonella enterica serotype Enteritidis: increasing incidence of domestically acquired infections / Clin. Infect. Dis 54 : S488 ~ S497
Collins BS. / 2011 / Gram-negative outer membrane vesicles in vaccine development / Discov. Med 12 : 7 ~ 15
De Cort W / 2013 / A Salmonella Enteritidis hilAssrAfliG deletion mutant is a safe live vaccine strain that confers protection against colonization by Salmonella Enteritidis
Bonnington KE / 2014 / Protein selection and export via outer membrane vesicles / Biochim. Biophys. Acta 1843 : 1612 ~ 1619
Chai SJ / 2012 / Salmonella enterica serotype Enteritidis: increasing incidence of domestically acquired infections / Clin. Infect. Dis 54 : S488 ~ S497
Collins BS. / 2011 / Gram-negative outer membrane vesicles in vaccine development / Discov. Med 12 : 7 ~ 15
De Cort W / 2013 / A Salmonella Enteritidis hilAssrAfliG deletion mutant is a safe live vaccine strain that confers protection against colonization by Salmonella Enteritidis
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