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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제5호
- 발행연도
- 2016.1
- 수록면
- 918 - 927 (10page)
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초록· 키워드
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Cryptococcus neoformans is a life-threatening pathogenic yeast that causes devastating meningoencephalitis. The mechanism of cryptococcal brain invasion is largely unknown, and recent studies suggest that its extracellular microvesicles may be involved in the invasion process. The 14-3-3 protein is abundant in the extracellular microvesicles of C. neoformans, and the 14-3-3-GFP fusion has been used as the microvesicle’s marker. However, the physiological role of 14-3-3 has not been explored. In this report, we have found that C. neoformans contains a single 14-3-3 gene that apparently is an essential gene. To explore the functions of 14-3-3, we substituted the promoter region of the 14-3-3 with the copper-controllable promoter CTR4. The CTR4 regulatory strain showed an enlarged cell size, drastic changes in morphology, and a decrease in the thickness of the capsule under copper-enriched conditions. Furthermore, the mutant cells produced a lower amount of total proteins in their extracellular microvesicles and reduced adhesion to human brain microvascular endothelial cells in vitro. Proteomic analyses of the protein components under 14-3-3-overexpressed and -suppressed conditions revealed that the 14-3-3 function(s) might be associated with the microvesicle biogenesis. Our results support that 14-3-3 has diverse pertinent roles in both physiology and pathogenesis in C. neoformans. Its gene functions are closely relevant to the pathogenesis of this fungus.
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참고문헌 (42)
참고문헌 신청
Berndsen CE / 2008 / Molecular functions of the histone acetyltransferase chaperone complex Rtt109-Vps75 / Nat. Struct. Mol. Biol 15:948~956
Bridges D / 2005 / 14-3-3 Proteins: a number of functions for a numbered protein / Sci. STKE :re10
Chang YC / 2004 / Cryptococcal yeast cells invade the central nervous system via transcellular penetration of the blood-brain barrier / Infect. Immun 72:4985~4995
Chang YC / 2006 / CPS1, a homolog of the Streptococcus pneumoniae type 3polysaccharide synthase gene, is important for the pathobiology of Cryptococcus neoformans / Infect. Immun 74:3930~3938
Chen HM / 2003 / Cryptococcus neoformans induces alterations in cytoskeleton of human brain microvascular endothelial cells / J. Med. Microbiol 52:961~970
Bridges D / 2005 / 14-3-3 Proteins: a number of functions for a numbered protein / Sci. STKE :re10
Chang YC / 2004 / Cryptococcal yeast cells invade the central nervous system via transcellular penetration of the blood-brain barrier / Infect. Immun 72:4985~4995
Chang YC / 2006 / CPS1, a homolog of the Streptococcus pneumoniae type 3polysaccharide synthase gene, is important for the pathobiology of Cryptococcus neoformans / Infect. Immun 74:3930~3938
Chen HM / 2003 / Cryptococcus neoformans induces alterations in cytoskeleton of human brain microvascular endothelial cells / J. Med. Microbiol 52:961~970
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