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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제9호
- 발행연도
- 2015.1
- 수록면
- 1,493 - 1,501 (9page)
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초록· 키워드
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A Bacillus species, EMD4, with strong antibacterial activity was isolated from ganjang (soy sauce) and identified as B. subtilis. B. subtilis EMD4 strongly inhibited the growth of B. cereus ATCC14579 and B. thuringiensis ATCC33679. The antibacterial activity was stable at pH 3-9 but inactive at pH 10 and above. The activity was fully retained after 15 min at 80°C but reduced by 50% after 15 min at 90°C. The activity was completely destroyed by proteinase K and protease treatment, indicating its proteinaceous nature. The bacteriocin (BacEMD4) was partially purified from culture supernatant by ammonium sulfate precipitation, and QSepharose and Sephadex G-50 column chromatographies. The specific activity was increased from 769.2 AU/mg protein to 8,347.8 AU/mg protein and the final yield was 12.6%. The size of BacEMD4 was determined to be 3.5 kDa by Tricine SDS-PAGE. The N-terminal amino acid sequence was similar with that of Subtilosin A. Nucleotide sequencing of the cloned gene confirmed that BacEMD4 was Subtilosin A. BacEMD4 showed bactericidal activity against B. cereus ATCC14579.
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참고문헌 (22)
참고문헌 신청
Abdel-Mohsein H / 2010 / Isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne patho
Babasaki K / 1985 / Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: isolation, structural analysis, and biogenesis / J. Biochem 98 : 585 ~ 603
Bizani D / 2002 / Characterization of a bacteriocin produced by a newly isolated Bacillus sp. strain 8 A / J. Appl. Microbiol 93 : 512 ~ 519
Bradford MM / 1976 / Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Cherif A / 2008 / Characterization and partial purification of entomocin 110, a newly identified bacteriocin from Bacillus thuringiensis subsp. Entomocidus HD110 / Microbiol.
Babasaki K / 1985 / Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: isolation, structural analysis, and biogenesis / J. Biochem 98 : 585 ~ 603
Bizani D / 2002 / Characterization of a bacteriocin produced by a newly isolated Bacillus sp. strain 8 A / J. Appl. Microbiol 93 : 512 ~ 519
Bradford MM / 1976 / Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Cherif A / 2008 / Characterization and partial purification of entomocin 110, a newly identified bacteriocin from Bacillus thuringiensis subsp. Entomocidus HD110 / Microbiol.
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