지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제9호
- 발행연도
- 2015.1
- 수록면
- 1,401 - 1,409 (9page)
이용수
초록· 키워드
오류제보하기
The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYBR Green-based real-time PCR assay targeting the YD-repeat protein gene of A. avenae subsp. citrulli was developed. The specificity of the primer set was evaluated using DNA purified from 6 isolates of A. avenae subsp. citrulli, 7 other Acidovorax species, and 22 of non-targeted strains, including pathogens and non-pathogens. The AC158F/R primer set amplified a single band of the expected size from genomic DNA obtained from the A. avenae subsp. citrulli strains but not from the genomic DNA of other Acidovorax species, including that of other bacterial genera. Using this assay, it was possible to detect at least one genomeequivalents of the cloned amplified target DNA using 5 × 100 fg/μl of purified genomic DNA per reaction or using a calibrated cell suspension, with 6.5 colony-forming units per reaction being employed. In addition, this assay is a highly sensitive and reliable method for identifying and quantifying the target pathogen in infected samples that does not require DNA extraction. Therefore, we suggest that this approach is suitable for the rapid and efficient diagnosis of A. avenae subsp. citrulli contaminations of seed lots and plants.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Atlas RM / 1888 / Handbook of Microbiological Media / CRC Press : 913 ~ 1888
Bahar O / 2008 / New subspecies-specific polymerase chain reactionbased assay for the detection of Acidovorax avenae subsp. citrulli / Plant Pathol 57 : 754 ~ 763
Burdman S / 2005 / Molecular, physiological, and host-range characterization of Acidovorax avenae s ubsp. citrulli isolates from watermelon and melon in Israel / Plant Dis 89
Cho MS / 2011 / Sensitive and specific detection of Xanthomonas oryzae pv. oryzae by real-time bio-PCR using pathovar-specific primers based on an rhs family gene / Plant Dis
DeBoer S / 2007 / Biotechnology and Plant Disease Management / CAB International : 321 ~ 325
Bahar O / 2008 / New subspecies-specific polymerase chain reactionbased assay for the detection of Acidovorax avenae subsp. citrulli / Plant Pathol 57 : 754 ~ 763
Burdman S / 2005 / Molecular, physiological, and host-range characterization of Acidovorax avenae s ubsp. citrulli isolates from watermelon and melon in Israel / Plant Dis 89
Cho MS / 2011 / Sensitive and specific detection of Xanthomonas oryzae pv. oryzae by real-time bio-PCR using pathovar-specific primers based on an rhs family gene / Plant Dis
DeBoer S / 2007 / Biotechnology and Plant Disease Management / CAB International : 321 ~ 325
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction
The Plant Pathology Journal
2016 .12
-
박과 작물에 과일썩음병을 일으키는 Acidovorax citrulli 검출을 위한 nested-PCR 검사법 개발
Research in Plant Disease(식물병연구)
2015 .06
-
Complete genome sequence of Acidovorax citrulli strain KACC17005, a causal agent for bacterial fruit blotch on watermelon
미생물학회지
2017 .12
-
First Isolation and Molecular Characterization of Bacteriophages Infecting Acidovorax citrulli, the Causal Agent of Bacterial Fruit Blotch
The Plant Pathology Journal
2018 .02
-
국내 박과 작물에서 과실썩음병을 일으키는 Acidovorax citrulli 집단의 유전적 특성
Research in Plant Disease(식물병연구)
2015 .06
-
귀리 흑변 종자에서 분리된 Pyrenophora avenae의 특성
한국균학회지
2018 .01
-
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
The Plant Pathology Journal
2015 .06
-
나노 화합물을 이용한 Acidovorax citrulli 및 식물병원성 미생물의 항균활성 효과 검정
Research in Plant Disease(식물병연구)
2015 .03
-
저온플라즈마(Microwave Plasma)를 활용한 Acidovorax citrulli 감염 수박종자의 살균 효과 검정
Research in Plant Disease(식물병연구)
2021 .03
-
Natural Variation in Virulence of Acidovorax citrulli Isolates That Cause Bacterial Fruit Blotch in Watermelon, Depending on Infection Routes
The Plant Pathology Journal
2020 .02
-
수박에 과실썩음병을 유발하는 Acidovorax citrulli의 생물학적 방제를 위한 길항 미생물 선발과 특성 검정
Research in Plant Disease(식물병연구)
2022 .06
-
Development of Real-time PCR Assays for Detection of Dirofilaria immitis from Infected Dog Blood
대한임상검사과학회지
2016 .01
-
Biological Control of Bacterial Fruit Blotch of Watermelon Pathogen (Acidovorax citrulli) with Rhizosphere Associated Bacteria
The Plant Pathology Journal
2017 .04
-
Identification of a Cupin Protein Gene Responsible for Pathogenicity, Phage Susceptibility and LPS Synthesis of Acidovorax citrulli
The Plant Pathology Journal
2021 .12
-
Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains
The Plant Pathology Journal
2018 .04
-
Proteomic and Phenotypic Analyses of a Putative YggS Family Pyridoxal Phosphate-Dependent Enzyme in Acidovorax citrulli
The Plant Pathology Journal
2023 .06
-
Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR
Journal of Microbiology and Biotechnology
2015 .01
-
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
The Plant Pathology Journal
2015 .09
-
Cytogenetic Analysis of Bienertia sinuspersici Akhani as the First Step in Genome Sequencing
Genes & Genomics
2020 .01
-
대기입자상물질시료의곰팡이메타게놈분석을위한 DNA추출및 PCR조건최적화
한국미생물·생명공학회지
2023 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0