지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제12호
- 발행연도
- 2014.1
- 수록면
- 1,664 - 1,672 (9page)
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초록· 키워드
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In this study, a fibrous bed bioreactor (FBB) was used for D-lactic acid (D-LA) production by Sporolactobacillus inulinus Y2-8. Corn flour hydrolyzed with α-amylase and saccharifying enzyme was used as a cost-efficient and nutrient-rich substrate for D-LA production. A maximal starch conversion rate of 93.78% was obtained. The optimum pH for D-LA production was determined to be 6.5. Ammonia water was determined to be an ideal neutralizing agent, which improved the D-LA production and purification processes. Batch fermentation and fedbatch fermentation, with both free cells and immobilized cells, were compared to highlight the advantages of FBB fermentation. In batch mode, the D-LA production rate of FBB fermentation was 1.62 g/l/h, which was 37.29% higher than that of free-cell fermentation, and the D-LA optical purities of the two fermentation methods were above 99.00%. In fed-batch mode, the maximum D-LA concentration attained by FBB fermentation was 218.8 g/l, which was 37.67% higher than that of free-cell fermentation. Repeated-batch fermentation was performed to determine the long-term performance of the FBB system, and the data indicated that the average D-LA production rate was 1.62 g/l/h and the average yield was 0.98 g/g. Thus, hydrolyzed corn flour fermented by S. inulinus Y2-8 in a FBB may be used for improving D-LA fermentation by using ammonia water as the neutralizing agent.
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참고문헌 (22)
참고문헌 신청
Abdel-Rahman MA / 2010 / Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits / J. Biotechnol 156 : 286 ~ 301
Bai DM / 2004 / Ammonium lactate production by Lactobacillus lactis BME5-18M in pH-controlled fed-batch fermentations / Biochem. Eng. J 19 : 47 ~ 51
Datta R / 2006 / Lactic acid: recent advances in products, processes and technologies - a r eview / J. Chem. Technol. Biotechnol 81 : 1119 ~ 1129
Dien BS / 2001 / Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars / J. Ind. Microbiol. Biotechnol 27 : 259 ~ 264
Ding S / 2006 / L-Lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies / Process Biochem 41 : 1451 ~ 1454
Bai DM / 2004 / Ammonium lactate production by Lactobacillus lactis BME5-18M in pH-controlled fed-batch fermentations / Biochem. Eng. J 19 : 47 ~ 51
Datta R / 2006 / Lactic acid: recent advances in products, processes and technologies - a r eview / J. Chem. Technol. Biotechnol 81 : 1119 ~ 1129
Dien BS / 2001 / Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars / J. Ind. Microbiol. Biotechnol 27 : 259 ~ 264
Ding S / 2006 / L-Lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies / Process Biochem 41 : 1451 ~ 1454
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