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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제10호
- 발행연도
- 2014.1
- 수록면
- 1,427 - 1,437 (11page)
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초록· 키워드
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Enzymatic hydrolysis of lignocellulosic biomass into fermentable sugars is a key step in the conversion of agricultural by-products to biofuels and value-added chemicals. Utilization of a robust microorganism for on-site production of biomass-degrading enzymes has gained increasing interest as an economical approach for supplying enzymes to biorefinery processes. In this study, production of multi-polysaccharide-degrading enzymes from Aspergillus aculeatus BCC199 by solid-state fermentation was improved through the statistical design approach. Among the operational parameters, yeast extract and soybean meal as well as the nonionic surfactant Tween 20 and initial pH were found as key parameters for maximizing production of cellulolytic and hemicellulolytic enzymes. Under the optimized condition, the production of FPase, endoglucanase, β-glucosidase, xylanase, and β-xylosidase was achieved at 23, 663, 88, 1,633, and 90 units/g of dry substrate, respectively. The multi-enzyme extract was highly efficient in the saccharification of alkaline-pretreated rice straw, corn cob, and corn stover. In comparison with commercial cellulase preparations, the BCC199 enzyme mixture was able to produce remarkable yields of glucose and xylose, as it contained higher relative activities of β-glucosidase and core hemicellulases (xylanase and β-xylosidase). These results suggested that the crude enzyme extract from A. aculeatus BCC199 possesses balanced cellulolytic and xylanolytic activities required for the efficient saccharification of lignocellulosic biomass feedstocks, and supplementation of external β-glucosidase or xylanase was dispensable. The work thus demonstrates the high potential of A. aculeatus BCC199 as a promising producer of lignocellulose-degrading enzymes for the biomass conversion industry.
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참고문헌 (22)
참고문헌 신청
Badhan AK / 2007 / Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099 / Bioresour. Technol 98 : 504 ~ 510
Bagga PS / 1990 / Purification and characterization of cellulolytic enzymes produced by Aspergillus nidulans / J. Appl. Bacteriol 68 : 61 ~ 68
Bansal N / 2012 / Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues / Waste Manag 32 : 1341 ~ 1346
Bischoff K / 2009 / Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae / Curr. Microbiol 58 : 499 ~ 503
Chapla D / 2010 / Utilization of agro-industrial waste for xylanase production by Aspergillus foetidus MTCC 4898 under solid state fermentation and its application in sacchar
Bagga PS / 1990 / Purification and characterization of cellulolytic enzymes produced by Aspergillus nidulans / J. Appl. Bacteriol 68 : 61 ~ 68
Bansal N / 2012 / Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues / Waste Manag 32 : 1341 ~ 1346
Bischoff K / 2009 / Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae / Curr. Microbiol 58 : 499 ~ 503
Chapla D / 2010 / Utilization of agro-industrial waste for xylanase production by Aspergillus foetidus MTCC 4898 under solid state fermentation and its application in sacchar
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