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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제10호
- 발행연도
- 2014.1
- 수록면
- 1,445 - 1,454 (10page)
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초록· 키워드
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Severe acute respiratory syndrome (SARS), a disease that spread widely in the world during late 2002 to 2004, severely threatened public health. Although there have been no reported infections since 2004, the extremely pathogenic SARS coronavirus (SARS-CoV), as the causative agent of SARS, has recently been identified in animals, showing the potential for the re-emergence of this disease. Previous studies showed that 27 single nucleotide polymorphism (SNP) mutations among the spike (S) gene of this virus are correlated closely with the SARS pathogenicity and epidemicity. We have developed a SNP DNA microarray in order to detect and genotype these SNPs, and to obtain related information on the pathogenicity and epidemicity of a given strain. The microarray was hybridized with PCR products amplified from cDNAs obtained from different SARS-CoV strains. We were able to detect 24 SNPs and determine the type of a given strain. The hybridization profile showed that 19 samples were detected and genotyped correctly by using our microarray, with 100% accuracy. Our microarray provides a novel method for the detection and epidemiological surveillance of SARS-CoV.
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참고문헌 (24)
참고문헌 신청
Balboni A / 2012 / SARS-like coronaviruses: the role of bats and evolutionary relationships with SARS coronavirus / New Microbiol 35 : 1 ~ 16
Bosch BJ / 2003 / The coronavirus spike protein is a class I virus fusion protein:structural and functional characterization of the fusion core complex / J. Virol 77 : 8801 ~
Colman PM / 2003 / The structural biology of type I viral membrane fusion / Nat. Rev. Mol. Cell Biol 4 : 309 ~ 319
Drosten C / 2003 / Identification of a novel coronavirus in patients with severe acute respiratory syndrome / N. Engl. J. Med 348 : 1967 ~ 1976
Guan Y / 2003 / Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China / Science 302 : 276 ~ 278
Bosch BJ / 2003 / The coronavirus spike protein is a class I virus fusion protein:structural and functional characterization of the fusion core complex / J. Virol 77 : 8801 ~
Colman PM / 2003 / The structural biology of type I viral membrane fusion / Nat. Rev. Mol. Cell Biol 4 : 309 ~ 319
Drosten C / 2003 / Identification of a novel coronavirus in patients with severe acute respiratory syndrome / N. Engl. J. Med 348 : 1967 ~ 1976
Guan Y / 2003 / Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China / Science 302 : 276 ~ 278
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