지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제10호
- 발행연도
- 2014.1
- 수록면
- 1,397 - 1,404 (8page)
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초록· 키워드
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A few starters have been developed and used for doenjang fermentation but often without safety evaluation. Filamentous fungi were isolated from industrial doenjang koji, and their potential for mycotoxin production was evaluated. Two fungi were isolated; one was more dominantly present (90%). Both greenish (SNU-G) and whitish (SNU-W) fungi showed 97% and 95% internal transcribed spacer sequence identities to Aspergillus oryzae/flavus, respectively. However, the SmaI digestion pattern of their genomic DNA suggested that both belong to A. oryzae. Moreover, both fungi had morphological characteristics similar to that of A. oryzae. SNU-G and SNU-W did not form sclerotia, which is a typical characteristic of A. oryzae. Therefore, both fungi were identified to be A. oryzae. In aflatoxin gene cluster analysis, both fungi had norB-cypA genes similar to that of A. oryzae. Consistent with this, aflatoxins were not detected in SNU-G and SNU-W using ammonia vapor, TLC, and HPLC analyses. Both fungi seemed to have a whole cyclopiazonic acid (CPA) gene cluster based on PCR of the maoA, dmaT, and pks-nrps genes, which are key genes for CPA biosynthesis. However, CPA was not detected in TLC and HPLC analyses. Therefore, both fungi seem to be safe to use as doenjang koji starters and may be suitable fungal candidates for further development of starters for traditional doenjang fermentation.
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참고문헌 (23)
참고문헌 신청
Bae SM. / 2011 / Methods for manufacturing meju using mixed fungal strains / Korea patent application 10-2011-0126447
Chang PK / 2005 / Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates / Fungal Genet. Biol 42
Chang PK / 2006 / Cladal relatedness among Aspergillus oryzae isolates and Aspergillus flavus S and L morphotype isolates / Int. J. Food Microbiol 108 : 172 ~ 177
Chang PK / 2009 / Clustered genes involved in cyclopiazonic acid production are next to the aflatoxin biosynthesis gene cluster in Aspergillus flavus / Fungal Genet. Biol 46
Criseo G / 2008 / High genetic variability in non-aflatoxigenic A. flavus strains by using quadruplex PCR-based assay / Int. J. Food Microbiol 125 : 341 ~ 343
Chang PK / 2005 / Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates / Fungal Genet. Biol 42
Chang PK / 2006 / Cladal relatedness among Aspergillus oryzae isolates and Aspergillus flavus S and L morphotype isolates / Int. J. Food Microbiol 108 : 172 ~ 177
Chang PK / 2009 / Clustered genes involved in cyclopiazonic acid production are next to the aflatoxin biosynthesis gene cluster in Aspergillus flavus / Fungal Genet. Biol 46
Criseo G / 2008 / High genetic variability in non-aflatoxigenic A. flavus strains by using quadruplex PCR-based assay / Int. J. Food Microbiol 125 : 341 ~ 343
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