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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제8호
- 발행연도
- 2014.1
- 수록면
- 1,034 - 1,043 (10page)
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This study determined the effect of light intensity and photoperiod on the dry cell weight and total amount of carotenoids in four isolates of purple non-sulfur bacteria obtained from shaded and exposed microhabitats of a mangrove ecosystem in Kota Kinabalu, Sabah, Malaysia. The initial isolation of the bacteria was carried out using synthetic 112 medium under anaerobic conditions (2.5 klx) at 30 ± 2°C. On the basis of colony appearance, cell morphology, gram staining, motility test, and 16S rRNA gene sequencing analyses, all four bacteria were identified as Afifella marina. One of the bacterial isolates, designated as Af. marina strain ME, which was extracted from an exposed mud habitat within the mangrove ecosystem, showed the highest yield in dry cell weight (4.32± 0.03 g/l) as well as total carotenoids (0.783 ± 0.002 mg/g dry cell weight). These values were significantly higher than those for dry cell weight (3.77 ± 0.02g/l ) and total carotenoid content (0.706 ± 0.008 mg/g) produced by the isolates from shaded habitats. Further analysis of the effect of 10 levels of light intensity on the growth characteristics of Af. marina strain ME showed that the optimum production of dry cell weight and total carotenoids was achieved at different light intensities and incubation periods. The bacterium produced the highest dry cell weight of 4.98 g/l at 3 klx in 72 h incubation, but the carotenoid production of 0.783 mg/g was achieved at 2.5 klx in 48 h incubation. Subsequent analysis of the effect of photoperiod on the production of dry cell weight and total carotenoids at optimum light intensities (3 and 2.5 klx, respectively) revealed that 18 and 24 h were the optimum photoperiods for the production of dry cell weight and total carotenoids, respectively. The unique growth characteristics of the Af. marina strain ME can be exploited for biotechnology applications.
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참고문헌 (25)
참고문헌 신청
Ainon H / 2006 / Biological characterization of Rhodomicrobium vannielii isolated from a hot spring at Gadek, Malacca, Malaysia / Malays. J. Microbiol 2 : 15 ~ 21
Altschul SF / 1990 / Basic local alignment search tool / J. Mol. Biol 215 : 403 ~ 410
Asker D / 2007 / Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan / Appl. Microbiol. Cell Physiol 77 : 383 ~ 392
Ausich RL. / 1997 / Commercial opportunities for carotenoid production by biotechnology / Pure Appl. Chem 69 : 2169 ~ 2173
Azad SA / 2002 / Phototrophic bacteria as feed supplement for rearing Penaeus monodon larvae / J. World Aquacult. Soc 33 : 991 ~ 994
Altschul SF / 1990 / Basic local alignment search tool / J. Mol. Biol 215 : 403 ~ 410
Asker D / 2007 / Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan / Appl. Microbiol. Cell Physiol 77 : 383 ~ 392
Ausich RL. / 1997 / Commercial opportunities for carotenoid production by biotechnology / Pure Appl. Chem 69 : 2169 ~ 2173
Azad SA / 2002 / Phototrophic bacteria as feed supplement for rearing Penaeus monodon larvae / J. World Aquacult. Soc 33 : 991 ~ 994
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