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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제8호
- 발행연도
- 2014.1
- 수록면
- 1,109 - 1,117 (9page)
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초록· 키워드
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Chlorogenic acid and hydroxylcinnamoyl shikimates are major dietary phenolics as well as antioxidants, with recently discovered biological, activities including protection against chemotheraphy side effects and prevention of cardiovascular disease and cancer. Certain fruits and vegetables produce these compounds, although a microbial system can also be utilized for synthesis of chlorogenic acid and hydroxylcinnamoyl shikimates. In this study, we engineered Escherichia coli to produce chlorogenic acid and p-coumaroyl shikimates from glucose. For the synthesis of chlorogenic acid, two E. coli strains were used; one strain for the synthesis of caffeic acid from glucose and the other strain for the synthesis of chlorogenic acid from caffeic acid and quinic acid. The final yield of chlorogenic acid using this approach was approximately 78 mg/l. To synthesize p-coumaroyl shikimates, wild-type E. coli as well as several mutants were tested. Mutant E. coli carrying deletions in three genes (tyrR, pheA, and aroL) produced 236 mg/l of p-coumaroyl shikimates.
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참고문헌 (21)
참고문헌 신청
Clifford MN. / 1999 / Chlorogenic acids and other cinnamates - nature, occurrence and dietary burden / J. Sci. Food Agric 79 : 362 ~ 372
Dixon RA / 1995 / Stress-induced phenylpropanoid metabolism / Plant Cell 7 : 1085 ~ 1097
Draths KM / 1999 / Shikimic acid and quinic acid: replacing isolation from plant sources with recombinant microbial biocatalysis / J. Am. Chem. Soc 121 : 1603 ~ 1604
E l-Seedi HR / 2012 / Biosynthesis, natural sources, dietary intake, pharmacokinetic properties, and biological activities of hydroxycinnamic acids / J. Agric. Food Chem 60 :
E ly B / 1979 / Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12 / J. Bacterol 138 : 933 ~ 943
Dixon RA / 1995 / Stress-induced phenylpropanoid metabolism / Plant Cell 7 : 1085 ~ 1097
Draths KM / 1999 / Shikimic acid and quinic acid: replacing isolation from plant sources with recombinant microbial biocatalysis / J. Am. Chem. Soc 121 : 1603 ~ 1604
E l-Seedi HR / 2012 / Biosynthesis, natural sources, dietary intake, pharmacokinetic properties, and biological activities of hydroxycinnamic acids / J. Agric. Food Chem 60 :
E ly B / 1979 / Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12 / J. Bacterol 138 : 933 ~ 943
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