지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제10호
- 발행연도
- 2012.1
- 수록면
- 1,412 - 1,422 (11page)
이용수
초록· 키워드
오류제보하기
The aim of this work was to establish the optimal conditions for production of carboxymethylcellulase (CMCase) by a newly isolated marine bacterium using response surface methodology (RSM). A microorganism producing CMCase,isolated from seawater, was identified as Cellulophaga lytica based 16S rDNA sequencing and the neighborjoining method. The optimal conditions of rice bran,ammonium chloride, and initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively,whereas those for production of CMCase were 79.9 g/l,8.52 g/l, and 6.1. The optimal concentrations of K2HPO4,NaCl, MgSO4·7H2O, and (NH4)2SO4 for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for production of CMCase were 3.72, 0.54, 0.70, and 0.34 g/l. The optimal temperature for cell growth and the CMCase production by C. lytica LBH-14 were 35oC and 25oC, respectively. The maximal production of CMCase under optimized condition for 3 days was 110.8 U/ml,which was 5.3 times higher than that before optimization.
In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of CMCase by C. lytica LBH-14. The time for production of CMCase by a newly isolated marine bacterium with submerged fermentations reduced to 3 days, which resulted in enhanced productivity of CMCase and a decrease in its production cost.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Ballesteros, M. / 2004 / Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SSF) with Kluyveromeces marxianus CECT 10875 / Pr
Blumer-Schuette, S. E. / 2008 / Extremely thermophilic microorganisms for biomass conversion: Status and prospects / Curr. Opin. Biotechnol 19 : 210 ~ 217
CHAUHAN, KISHOR / 2006 / Application of Response Surface Methodology for Optimization of Lactic Acid Production Using Date Juice / Journal of Microbiology and Biotechnology 1
Chen, Hui / 2010 / Optimization of Hydroxyl Radical Scavenging Activity of Exopolysaccharides from Inonotus obliquus in Submerged Fermentation Using Response Surface Methodol
EMTIAZI, G / 2004 / Production of Thermostable α-Amylase and Cellulase from Cellulomonas sp. / Journal of Microbiology and Biotechnology 14 (6) : 1196 ~ 1199
Blumer-Schuette, S. E. / 2008 / Extremely thermophilic microorganisms for biomass conversion: Status and prospects / Curr. Opin. Biotechnol 19 : 210 ~ 217
CHAUHAN, KISHOR / 2006 / Application of Response Surface Methodology for Optimization of Lactic Acid Production Using Date Juice / Journal of Microbiology and Biotechnology 1
Chen, Hui / 2010 / Optimization of Hydroxyl Radical Scavenging Activity of Exopolysaccharides from Inonotus obliquus in Submerged Fermentation Using Response Surface Methodol
EMTIAZI, G / 2004 / Production of Thermostable α-Amylase and Cellulase from Cellulomonas sp. / Journal of Microbiology and Biotechnology 14 (6) : 1196 ~ 1199
이 논문의 저자 정보
최근 본 자료
댓글(0)
0